念珠菌性阴道炎阴道分泌物天然免疫成分的表达

Expression of innate immunity components of vaginal secretion in the patients with vulvo - vaginal candidiasis

目的:评价β-防御素(β-defence-2)、甘露糖凝集素(mannose-binding lectin,MBL)和Toll样受体家族(Toll-like receptor,TLR)天然免疫成分在念珠菌性阴道炎患者中的表达。方法:提取念珠菌性阴道炎患者及健康带菌者阴道分泌物的RNA,利用荧光定量PCR检测阴道分泌物中天然免疫成分的表达。结果:β-defence-2表达量在患者阴道炎分泌物标本中均显著升高。MBL在念珠菌性阴道炎患者及健康带菌者阴道分泌物标本间表达的差异无统计学意义。健康带菌者TLR3、TLR2和TLR4表达水平明显低于念珠菌性阴道炎患者。结论:念珠菌性阴道炎患者阴道分泌物中存在多种抗白念珠菌感染的天然免疫因子。

Objective： To evaluate the effect of innate immunity components including β - defensin - 2, mannose - binding lectin （MBL） and Tolllike receptor （TLRs） in the patients with vulvo- vaginal candidiasis. Method： The expression of innate immunity components in vaginal secretion was tested by real time PCR. Results： The expression of β - defensin - 2 was significantly higher than that in controls. There was no significant difference in the expression of MBL between the two groups. The expression of TLR3, TLR4 and TLR2 in controls was decreased significantly compared to the patients with vulvovaginal candidiasis. Conclusion： The results demonstrate that innate immunity mediated by several anti - candidial factors exists in vaginal secretion of the patients with vulvo - vaginal candidiasis.