胃癌组织中微RNA-34b/c和微RNA-124a基因的甲基化

DNA methylation of microRNA-34b/c and microRNA-124a in gastric cancer

目的 探讨DNA甲基化对微RNA（miRNA）表达的影响.方法 取2009年7至11月天津医科大学总医院新鲜活检标本胃癌组、对照组各40例.应用甲基化特异性PCR（MSP）方法检测组织中miRNA-34b/c和miRNA-124a基因启动子区甲基化.结果 miRNA-34b/c基因启动子区高甲基化阳性率在胃癌组及对照组胃黏膜中分别为77.5%（31/40）和5.0%（2/40）,差异有统计学意义（P〈0.05）.miRNA-124a基因启动子区高甲基化阳性率在两组中分别为60.0%（24/40）和0,差异有统计学意义（P〈0.05）.miRNA基因启动子区高甲基化阳性率与胃癌临床病理特征有关.结论 胃癌组织存在miRNA-34b/c和miRNA-124a基因启动子区高甲基化,并可能在胃癌的发生发展中起重要作用.

Objective To explore the effects of DNA methylation of microRNA （miRNA） gene on their expressions in patients with gastric carcinoma.Methods A total of 80 subjects were divided into gastric carcinoma group（n=40）and control group（n=40）.The DNA methylation status of miRNA-34b/c and miRNA-124a gene promoters was detected by DNA methylation specific polymerase chain reaction （MSP） in gastric carcinoma tissues and normal mucosal tissues.Results The positive rate of DNA methylation of miRNA-34b/c gene promoter was 77.5%（31/40）and 5.0%（2/40）in gastric carcinoma and control groups respectively.There was statistically significant difference between two groups （P〈0.05）. The positive rate of DNA methylation of miRNA-124a gene promoter was 60.0%（24/40）and 0 in these two groups respectively.There was statistically significant difference between two groups （P〈0.05）.Also the hypermethylation positivity of gene promoter of miRNAs was correlated with the clinicopathological features of gastric carcinoma.Conclusion The hypermethylation of miRNA-34b/c and miRNA-124a gene promoters may play a crucial role in the occurrence and development of gastric carcinoma.