核因子κB、基质金属蛋白酶3在退变腰椎间盘组织中的表达

Expression of nuclear factor kappa B and matrix metalloproteinases 3 in intervertebral disc degeneration

背景:对于椎间盘退变的原因一直是国内外学者研究的重点。近年来随着研究的深入,在椎间盘退变过程中多种细胞因子、黏附分子、趋化因子、炎症递质、蛋白质酶等发挥着重要作用。目的:比较正常腰椎间盘组织及退变腰椎间盘组织中核因子κB、基质金属蛋白酶3的表达,分析两者的相关性。方法:收集唐山市第二医院脊柱外科手术切除退变椎间盘组织68例(病变组),腰椎爆裂骨折正常椎间盘组织10例(对照组),应用苏木精-伊红染色、免疫组织化学方法和ELISA方法检测核因子κB、基质金属蛋白酶3的表达,并对两者相关性进行分析。结果与结论:苏木精-伊红染色结果显示对照组可见纤维环和髓核结构,腰椎间盘髓核的软骨细胞表现为不规则的软骨陷窝,病变组细胞增殖明显,细胞核呈圆形或卵圆形,髓核细胞胞质呈空泡状。免疫组织化学方法显示病变组核因子κB、基质金属蛋白酶3吸光度值明显高于对照组(P<0.05)。ELISA检测病变组核因子κB、基质金属蛋白酶3的表达均高于对照组(P<0.05)。两者具有正相关性(r=0.6430,P<0.01)。

BACKGROUND： During intervertebral disc degeneration, various cytokines, adhesion molecules, chemokines, inflammatory neurotransmitters, protein enzymes play an important role. OBJECTIVE： To study the expression and significance of nuclear factor kappa B （NF-KB） and matrix metalloproteinases 3 （MMP-3） in normal disc tissue and intervertebral disc degeneration. METHODS： Sixty-eight patients with intervertebral disc degeneration from surgical intervention for cervical disc herniation （lesion group） and 10 cases of normal disc tissue （control group） were included. The expressions of NF-KB and MMP-3 were tested by hematoxylin-eosin staining, immunohistochemistry method and ELISA. RESULTS AND CONCLUSION： The NF-KB and MMP-3 positive expression rate was higher in the control group （P 〈 0.05）. There was a positive correlation （r=0.643 0, P 〈 0.01 ）. It indicated that NF-KB and MMP-3 may have important effects on intervertebral disc degeneration.