兔骨内高压型膝关节骨性关节炎模型的建立

Construction of a high intraosseous pressure type rabbit osteoarthritis model

背景:虽然实验中使用的膝关节骨性关节炎动物模型众多,但研究中医手法作用机制所采用的动物模型应能承受"动"的作用并发挥出正常"动"产生的效应。目的:确定建立骨内高压型膝关节骨性关节炎动物模型的效果。方法:无菌条件下显露新西兰兔右侧臀部的臀下静脉,右下腹部的股静脉和大隐静脉,结扎并切断;以同样手术显露相应血管但不结扎切断血管的动物及正常组动物作为对照。术后8周末处死所有动物,取动物右膝内侧胫骨平台软骨组织通过大体观察及苏木精-伊红染色、光镜观察软骨组织的结构变化,评估动物模型的造模效果。结果与结论:模型组关节面粗糙、灰黄色,可见明显缺损及骨赘形成,达到初中期变化。假模组关节面粗糙,灰白色无光泽,处于早期变化。正常组关节面光滑,边缘规整,软骨半透明有光泽,无变化。结果提示,通过结扎动物臀下静脉、股静脉及大隐静脉8周后,可形成早中期膝关节骨性关节炎动物模型。

BACKGROUND：Plenty of animal models of knee osteoarthritis has been established and used,most of them may not be right for the experimental study of Chinese medicine manipulation.OBJECTIVE：To determine the outcomes of constructed animal models of high intraosseous pressure knee osteoarthritis.METHODS：The inferior gluteal vein,femoral vein and great saphenous vein on the right side were exposed under aseptic conditions,ligatured and cutting off the veins.The animal with exposed vessel and normal animals were served as controls.All animals were sacrificed at 8 weeks after modeling.The structure changes of cartilage tissues were observed by gross observation,haematoxylin-eosin staining,and light microscope.RESULTS AND CONCLUSION：Articular surface of the model group was rough and dark-black,with obvious defects and osteophytosis,with the early and medial stages of osteoarthritis.Articular surface of sham-surgery group was rough,opacity and grey,with the early stages of osteoarthritis.Articular surface of the normal group was smooth,marginal regular,translucent shine for articular cartilage,with no obvious cartilage degeneration.The results revealed that,at 8 weeks after ligaturing the inferior gluteal vein,femoral vein and great saphenous vein would cause the early and medial stages of osteoarthritis in rabbits.