脊髓损伤模型大鼠神经胶质细胞增殖与胶质纤维酸性蛋白的表达

Proliferation of glial cells and expression of glial fibrillary acidic protein after spinal cord injury in rats

背景:星形胶质细胞可以通过细胞裂解释放各种神经营养因子,并可促进损伤脊髓的修复。目的:观察脊髓损伤模型大鼠神经胶质纤维酸性蛋白的表达及对其后肢功能恢复的影响。方法:将SD大鼠采用Allen's法撞击T9～10节段致脊髓损伤,造模成功后蛛网膜下腔移植骨形态发生蛋白7,并设置仅蛛网膜下腔移植His蛋白的正常SD大鼠做对照。用BBB评分法评估两组大鼠后肢的运动功能,用免疫组织化学染色法和Western-blot法观察各组神经胶质纤维酸性蛋白的表达。结果与结论:BBB评分结果显示,模型组大鼠脊髓损伤后下肢功能自行恢复率达68%。模型组脊髓损伤3和7d,损伤区域神经胶质纤维酸性蛋白表达逐渐增加(P<0.05),随后逐渐下降,于脊髓损伤28d后逐渐恢复到对照组水平(P>0.05)。脊髓损伤后1～14d两组胶质纤维酸性蛋白表达逐渐升高(P>0.05)。结果证实,脊髓损伤后蛛网膜下腔移植骨形态发生蛋白7可诱导星形胶质细胞增殖,神经胶质纤维酸性蛋白的表达增强,进而促进脊髓损伤大鼠后肢功能的恢复。

BACKGROUND：Astrocytes can release various neurotrophic factors by cell lysis to promote spinal cord repair.OBJECTIVE：To observe the recovery of hjndlimb function and the expression of glial fibrillary acidic protein （GFAP） after spinal cord injury.METHODS：Spinal cord injury models at T9-10 were established with Allen＇s method.After modeling,bone morphogenetic protein 7 was injected into the subarachnoid space.Normal SD rats with His protein injected into the subarachnoid space were used as controls.The behavioral evaluation was performed with the Basso,Beattie Bresnahanlocomotor rating scale （BBB scale）.GFAP expression was detected using hematoxylin-eosin staining and immunohistochemistry staining.RESULTS AND CONCLUSION：Based on BBB score,the hindlimb function of rats in the model group recovered 68%.GFAP expression in the model group increased gradually at 3 and 7 days after injury （P〈0.05）,and then decreased similar to the control group at 28 days after injury （P〈0.05）.The data indicated that injection of bone morphogenetic protein 7 into the subarachnoid space can induce the proliferation of glial cells,increase GFAP expression and then to promote the recovery of hindlimb function after spinal cord injury in rats.