不同质量浓度阿仑膦酸钠干预牙槽骨吸收模型兔牙周组织破骨细胞分化因子和骨保护素的表达

Expression of receptor activator of nuclear factor-kappa B ligand/osteoprotegerin in periodontal tissues in rabbits with periodontitis in response to alendronate

背景:有研究表明阿仑膦酸钠能够防治骨质疏松症,但其应用到口腔牙周组织干预牙槽骨吸收较为罕见。目的:建立兔牙槽骨吸收模型,观察不同质量浓度阿仑膦酸钠干预下炎症牙周组织破骨细胞分化因子和骨保护素的表达。方法:将大耳白兔建立牙槽骨吸收模型,建模成功后随机分成5组,胶原+0.5g/L阿仑膦酸钠组、胶原+1g/L阿仑膦酸钠组、胶原+2g/L阿仑膦酸钠组、胶原组、对照组。各组分别于用药后2和4周用免疫组织化学方法检测破骨细胞分化因子和骨保护素表达情况,并进行药效评价。结果与结论:破骨细胞分化因子和骨保护素阳性细胞在5组成骨细胞、破骨细胞、成纤维细胞中均有表达,胞浆呈棕色或棕褐色颗粒状着色。对照组和胶原组牙槽嵴吸收区破骨细胞分化因子和骨保护素比率明显大于其他3组(P<0.05),胶原+1g/L阿仑膦酸钠组在用药后2和4周均高于胶原+0.5g/L阿仑膦酸钠组(P<0.05)。结果证实,阿仑膦酸钠能够降低牙槽骨破骨细胞分化因子和骨保护素的比率,应用含1g/L阿仑膦酸钠胶原海绵载体更适合抑制牙槽骨吸收。

BACKGROUND：Studies have shown that alendronate can prevent and treat osteoporosis,but there are few reports about alendronate application for alveolar bone absorption.OBJECTIVE：To build an animal model of alveolar bone absorption so as to evaluate the expression of receptor activator of nuclear factor-kappa B ligand （RANKL）/osteoprotegerin （OPG） expression in periodontal tissues with periodontitis in response to different concentrations of alendronate.METHODS：Rabbit models were divided into five groups：collagen＋0.5 g/L alendronate group,collagen＋1 g/L alendronate group,collagen＋2 g/L alendronate group,collagen group and control group.The expression of RANKL and OPG in the periodontal tissues was detected with immunohistochemistry method.RESULTS AND CONCLUSION：RANKL and OPG were localized in osteoblasts,osteoclasts and fibroblasts.RANKL/OPG ratio in the control and collagen groups was significantly greater than that in the other three groups （P〈0.05）.RANKL/OPG ratio in the collagen＋1 g/L alendronate group was higher than that in the collagen＋0.5 g/L alendronate group at 2 and 4 weeks after medication （P 0.05）.The results indicated that alendronate,especially at a concentration of 1 g/L could decrease RANKL/OPG ratio and inhibit alendronate alveolar bone absorption.