声振表面活性剂微泡制备中温度及其他因素间的相互影响

Interaction between temperature and other factors in preparation of sonicated surfactant microbubbles

目的优化表面活性剂微泡的制备工艺,探讨溶液温度、声振功率及声振时间对表面活性剂微泡制备的相互影响。方法以一定质量比的山梨糖醇酐单硬脂酸酯(Span)和聚氧乙烯脱水山梨醇单油酸酯(Tween)溶解于磷酸盐缓冲液。将溶液分为控温组与非控温组,以不同声振功率和声振时间对两组溶液进行声振处理。于光镜下观察两组溶液所产微泡的大小和形态,测量其浓度。选用优化条件下生产的微泡对新西兰大白兔进行耳缘静脉注射,剂量为0.1 ml/kg,观察肝实质增强程度及持续时间。结果表面活性剂微泡的最佳制备条件为:控温条件下,声振功率800 W,声振时间90 s。注射造影剂后兔肝实质回声强度明显增强,120 s达到高峰,增强持续时间约10 min。结论通过控制温度,使用更高声振功率、更长声振时间,可以制备更具良好显影效果的微泡。

Objective To optimize the preparation process of surfactant microbubbles, and to explore the effects of solution temperatures, sonicated power and time on preparation of microbubbles. Methods A certain quality of Span and Tween were dissolved in PBS buffer solution. The solution was divided into temperature - controlled group and non - temperature controlled group. The two groups were respectively sonicated with different power and time. The size and shape of microbubbles were observed under the microscope, and the concentrations were measured by blood cell counting plate. Then the optimized microbubbles were intravenously injected into the rabbits with the dosage of 0.1 ml/kg, the enhancement and persistent time in liver parenchyma of the rabbits were observed. Results With the condition of temperature - control, 800 W sonicated power and 90 s sonicated time, the optimized surfactant microbubbles were prepared, which could significantly enhance the liver parenchyma echo intensity, the time to peak enhancement was 120 s, and the persistent time was approximately 10 min. Conclusion Higher yield surfactant microbubbles with better enhancement effect could be prepared＇by temperature - control with higher sonicated intensity and longer sonicated time.