5-氨基水杨酸对TNBS结肠炎大鼠中TL1A、NF-κB表达的影响

Effects on 5-ASA on the Expressions of TL1A and Nuclear Factor-Kappa B in TNBS-Induced Rat Colitis

目的:通过检测三硝基环磺酸(TNBS)诱导的结肠炎大鼠结肠组织中TL1A、NF-κB的表达及5-氨基水杨酸(5-ASA)干预后的表达,探讨它们和克罗恩病(CD)之间的关系及5-ASA干预后的影响。方法:选用雌性健康Wistar大鼠30只,均分为A、B、C组。A为正常对照组,B、C两组大鼠采用TNBS/乙醇灌肠制作大鼠结肠炎模型。造模后,B组每天给予0.9%氯化钠溶液1ml灌肠;C组每天给予5-ASA 1ml灌肠(100mg/kg)。于造模后第7天处死所有大鼠,按疾病活动指数(DAI)的评分标准进行大体损伤评分,HE染色进行组织损伤评分。同时取结肠病变部位组织,生化法检测MPO活性,应用荧光定量PCR检测TL1A、NF-κB的表达的变化。结果:与A组比较,B组和C组的DAI评分、大体损伤形态和组织学损伤评分及MPO活性均升高(P<0.05),但B组高于C组(P<0.05)。与A组相比,B、C组的TL1A、NF-κB水平升高(P<0.05),分别为0.09±0.51比0.91±0.17和0.35±0.05、0.11±0.06比0.82±0.17和0.33±0.14,且B组高于C组。结论:在TNBS诱导的大鼠炎性肠病模型中TL1A、NF-κB的表达增高,5-ASA对肠道炎症的治疗作用可能是通过抑制TL1A、NF-κB实现的。

Objective：To investigate the relationship among TL1A,NF-κB,and colitis,and the therapeutic effects of 5-aminosalicylic acid（5-ASA） on colitis by investigating the expression of TL1A and NF-κB in TNBS-induced rat colitis.Methods：Thirty female Wistar rats were randomly divided into three groups with 10 rats in each.Group A was served as normal control.The rats in group B and C were infused with TNBS/alcohol per rectum.After the induction of colitis,the rats in group C were treated daily with 1 ml of 5-ASA（100 mg/kg） per rectum,and those in group B were treated daily with 1 ml normal saline.All the animals were sacrificed at day 7 after induction of colitis.The macroscopic changes of the colon were evaluated according to disease activity index（DAI） scoring and histological change was assessed by HE staining.MPO activity of the mucosa was detected by biochemical methods.Expressions of TL1A and NF-κB in tissue were detected by Real-Time PCR.Results：Compared with that respectively in group A,DAI,macroscopic and histological scores,and MPO activity were significantly increased both in group B and C（P0.05）,and was higher in group B versus group C（P0.05）.The expressions of TL1A and NF-κB were different among the three groups（P0.05）,and both were the highest in group B and the lowest in group A.Conclusion：The expressions of TL1A and NF-κB in TNBS-induced rat colitis are increased.The 5-ASA therapeutic effects on colitis are possible achieved by inhibiting TL1A and NF-κB.