摘要
对从土壤中筛选到的酵母菌Y-9601 株进行了形态培养特征及生理生长特性的研究.该菌株以多边芽殖方式进行无性繁殖,形成子囊孢子.在无维生素培养基上不生长.同化和发酵萄葡糖、半乳糖、蔗糖、麦芽糖、1/3 棉子糖,不同化乳糖及硝酸钾.同化淀粉、纤维二糖及D-木糖能力强,对淀粉具有较强的酒精发酵能力,鉴定为酵母属糖化酵母种.其最适生长温度为30~34 ℃,最适生长pH 值为5.0~6.0,在麦芽汁中液体培养倍增时间为90 m in.
The morphology,cultural characteristics and physiological feature of the yeast Y 9601, which was isolated from soil, were studied. The strain reproduced vegetatively by budding multilatorally, forming ascospores. It could assimilate and ferment glucose,galactose, sucrose,maltose and 1/3 raffinose respectively, but couldn't assimilate lactose, potassium nitrate or grow on vitamin free medium. It has a strong ability to assimilate starch, cellbiose and D xylose and a high ethanol productivity from starch. The strain was identified as a species of Saccharomyces diastaticus. The optimal growth pH and temperature were 5.0~6.0and 30~34 ℃.The doubling time is 90 minutes in the wort liquid culture.
基金
江苏省环保资助!项目(编号98007)