Th17细胞在小鼠变应性鼻炎模型中的表达及调控作用

The expression and regulation of Th17 cell in murine modal of allergic rhinitis

目的:通过探讨变应性鼻炎小鼠与正常小鼠、抗IL-17抗体治疗后的变应性鼻炎小鼠的行为学、组织病理学,Th1、Th2、Th17细胞因子的数量以及Th17细胞特异性转录因子的表达水平,评价Th17细胞在变应性鼻炎发病机制中的作用。方法:以卵清蛋白致敏模型组和治疗组小鼠建立BALB/c小鼠变应性鼻炎模型,造模成功后治疗组予IL-17抗体治疗。对照组以生理盐水对照。采集外周血ELISA法测IL-17、IL-4和IFN-γ。并取黏膜行苏木精-伊红染色及RT-PCR法测孤儿核受体(RORγt)mRNA的表达水平。结果:卵清蛋白造模成功,治疗后症状及炎症病理缓解。模型组IL-17、IL-4及RORγt mRNA表达水平均较对照组明显增高,IFN-γ降低;治疗后前三者降低而后者升高(P<0.05)。结论:Th17细胞与Th2细胞在变应性鼻炎中大量表达,说明两者可能与其发病机制有关,本实验从IL-17抗体局部治疗有效方面提出治疗依据,通过调控Th17细胞可能成为有效阻止变应性鼻炎的治疗方法。

Objective：To evaluate the function of Th17 cells in allergic rhinitis,through comparing the symptoms,pathology and and the quantity of Th1,Th2 and Th17 cytokine in normal mice,allergic rhinitis mice and allergic rhinitis mice with IL-17 antibody application.Method：Thirty BALB/c mice were randomly divided into three groups,control group,allergic rhinitis group,and therapy group.The allergic rhinitis model was induced by classical method with ovalbumin.The therapy group was treated with IL-17 antibody.The concentration of IL-17,IL-4 and IFN-γ in serum was measured by enzyme-linked immunosorbent assay（ELISA）.Nasal mucosal inflammation was evaluated by HE staining.The expression of RORγt mRNA was measured by reverse transcription-polymerase chain reaction（RT-PCR）.Result：The expression level of IL-17,IL-4 and RORγt mRNA in allergic rhinitis group were significantly higher than those of control group and IL-17 antibody treated group（P0.05）.While the expression level of IFN-γ in allergic rhinitis group were significantly was lower than those of control group and IL-17 antibody treated group（P0.05）.The inflammation reaction in therapy group abated with nasal mucosal HE staining.Conclusion：The large quantity of Th2,Th17 cells were found in allergic rhinitis.It might be associated with the pathogenesis of allergic rhinitis.The control of Th17 cells expression may be an effective way to treat allergic rhinitis.