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雌激素对结肠癌细胞株COLO205错配修复基因表达的影响 被引量：2

Effect of estrogen on mismatch repair gene expression in colon cancer cells
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摘要 目的：研究雌激素对结肠癌细胞株COLO205错配修复基因表达的影响。方法：使用半定量RT-PCR和Westernblot,比较在不同浓度雌二醇（estradiol,E2）水平和雌激素受体拮抗剂ICI182.780处理前后结肠癌细胞株COLO205错配修复基因hMLH1和hMSH2的表达差异。并用real time RT-PCR进行验证。结果：E2能上调COLO205细胞株hMLH1基因的表达,这种上调效应发生在转录水平,在E2浓度为10-12~10-8mol/L范围内呈剂量依赖关系,且可被雌激素受体拮抗剂ICI182.780抑制;但E2对COLO205细胞的hMSH2表达没有明显影响。结论：雌激素能够增强结肠癌细胞株COLO205错配修复基因hMLH1的表达,这可能是雌激素预防结肠癌作用的一种新机制。 AIM：To investigate the effect of estrogen on MMR gene expression in colon cancer cells COLO205.METHODS：By employing semi-quantitative RT-PCR and Western blotting techniques,changes in the expression of MMR genes（hMLH1 and hMSH2） induced by different levels of estradiol（E2） and ICI182.780,an estrogen receptor inhibitor,was investigated in cultured COLO205 cells.The effect was then verified by real time RT-PCR.RESULTS：E2 enhanced the expression of hMLH1 in COLO205 cells at transcriptional level,and a dose-response relationship was established when the concentration of E2 was between 10-12～10-8 mol/L.The enhancement was suppressed by the estrogen receptor inhibitor ICI182.780.On the other hand,there was no significant effect of E2 on hMSH2 expression in COLO205 cells.CONCLUSION：E2 can increase the expression of hMLH1 in colon cancer cells COLO205,and this finding sheds new light on the mechanism of estrogen protecting against colon cancer by regulating MMR system.
作者 陆晓娟 余东亮 王佳锌 潘笑露 金鹏 李世荣 盛剑秋
机构地区 北京军区总医院消化内科 第三军医大学研究生院 贵阳医学院医学影像系
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2011年第7期754-756,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金资助项目(81072041)
关键词 结肠癌 雌激素 错配修复系统(MMR) HMLH1 HMSH2 colon cancer estrogen mismatch repair hMLH1 hMSH2
分类号 R392.11 [医药卫生—免疫学]
  • 相关文献

参考文献13

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二级参考文献13

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共引文献2

同被引文献24

  • 1Froggatt NJ,Green J,Brassett C,et al.A common MSH2mutation in English and North American HNPCC families:origin,phenotypic expression,and sex specific differences incolorectal cancer[J].J Med Genet,1999,36:97-102.
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  • 4Jin P,Lu XJ,Sheng JQ,et al.Estrogen stimulates the ex-pression of mismatch repair gene hMLH1 in colonic epitheli-al cells[J].Cancer Prev Res,2010,3:910-916.
  • 5Park JG,Kim DW,Hong CW,et al.Germ line mutationsof mismatch repair genes in hereditary nonpolyposis colorec-tal cancer patients with small bowel cancer:InternationalSociety for Gastrointestinal Hereditary Tumours Collabora-tive Study[J].Clin Cancer Res,2006,12:3389-3393.
  • 6Chlebowski RT,Wactawski-Wende J,Ritenbaugh C,et al.Estrogen plus progestin and colorectal cancer in postmenopaus-al women[J].N Engl J Med,2004,350:991-1004.
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  • 8Irving JA,Beverley D,Wang L,et al.Cyclical expressionof the DNA mismatch repair enzyme hMLH1 in normal en-dometrium[J].Fertil Steril,2002,78:195-196.
  • 9Chan JA,Meyerhardt JA,Chan AT,et al.Hormone replacement therapy and survival after colorectal cancer diagnosis[J].J Clin Oncol,2006,24(36):5680-5686.
  • 10Jin P,Lu XJ,Sheng JQ,et al.Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells[J].Cancer Prev Res(Phila),2010,3(8):910-916.

引证文献2

二级引证文献2

细胞与分子免疫学杂志
2011年 第7期

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