慢性病毒性肝炎患者肝组织转化生长因子β_1及其mRNA分布的研究

Expression of TGFb1 and its mRNA in liver tissiues of patients with chronic viral hepatitis

目的了解慢性病毒性肝炎患者肝组织转化生长因β１（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒｅｌ，ＴＧＦβ１）蛋白及ＴＧＦβｌｍＲＮＡ的分布状况及其意义。方法用免疫组织化学方法检测了４５例慢性病毒性肝炎患者肝组织ＴＧＦβ１蛋白表达，并做其中２１例ＴＧＦβ１ｍＲＮＡ原位杂交检测。结果ＴＧＦβ１蛋白肝组织表达阳性率为８８８９％，主要为间质细胞、汇管区及纤维化发生活跃部位阳性，且随肝纤维化程度加重而表达增强（ｒ＝０７３，Ｐ＜００５）；ＴＧＦβｌｍＲＮＡ检测阳性率为５７．１４％，不仅定位于间质细胞，某些肝细胞也有阳性信号。结论ＴＧＦβｌ与肝纤维化发生发展密切相关。在病毒性肝炎，不仅间质细胞能够产生ＴＧＦβ１，肝细胞本身也能分泌ＴＧＦβ１。

Objective To investigate the relationship between expression of transforming growthfactor 1(TGF1), TGF1 mRNA in liver tissue of patients with chronic viral hepatitis and degree of liverfibrosis. Mathods TGF1 protein was detected in 45 patients by immunohistochemistry and TGF1 mRNAwas detected in 21 of the 45 cases by in situ hybridization. Results TGF1 protein was 88.89%(40 of 45)positive in their liver tissues and was mainly distributed in interstitial cells, portal areas and the areaswhere fibrosis actively occured, and the expression of TGF1 protein was positively correlated with degreeof liver fibrosis(r=0.73, P<0.05). TGF1 mRNA was 80.95%(19 of 21)positive in their liver tissues. PositiveStains were not only detected in sinusoidal cells and mononuclear cells, but also in some parenchymal cells.Conclusions The expression of TGF61 correlated closely with liver fibrosis of viral hepatitis. Not ohlynit6rstitinl Cells, but also parenchymal cells in liver might participate in the fibrogenisis by producingTGF1.