摘要
目的了解慢性病毒性肝炎患者肝组织转化生长因β1(transforminggrowthfactorel,TGFβ1)蛋白及TGFβlmRNA的分布状况及其意义。方法用免疫组织化学方法检测了45例慢性病毒性肝炎患者肝组织TGFβ1蛋白表达,并做其中21例TGFβ1mRNA原位杂交检测。结果TGFβ1蛋白肝组织表达阳性率为8889%,主要为间质细胞、汇管区及纤维化发生活跃部位阳性,且随肝纤维化程度加重而表达增强(r=073,P<005);TGFβlmRNA检测阳性率为57.14%,不仅定位于间质细胞,某些肝细胞也有阳性信号。结论TGFβl与肝纤维化发生发展密切相关。在病毒性肝炎,不仅间质细胞能够产生TGFβ1,肝细胞本身也能分泌TGFβ1。
Objective To investigate the relationship between expression of transforming growthfactor 1(TGF1), TGF1 mRNA in liver tissue of patients with chronic viral hepatitis and degree of liverfibrosis. Mathods TGF1 protein was detected in 45 patients by immunohistochemistry and TGF1 mRNAwas detected in 21 of the 45 cases by in situ hybridization. Results TGF1 protein was 88.89%(40 of 45)positive in their liver tissues and was mainly distributed in interstitial cells, portal areas and the areaswhere fibrosis actively occured, and the expression of TGF1 protein was positively correlated with degreeof liver fibrosis(r=0.73, P<0.05). TGF1 mRNA was 80.95%(19 of 21)positive in their liver tissues. PositiveStains were not only detected in sinusoidal cells and mononuclear cells, but also in some parenchymal cells.Conclusions The expression of TGF61 correlated closely with liver fibrosis of viral hepatitis. Not ohlynit6rstitinl Cells, but also parenchymal cells in liver might participate in the fibrogenisis by producingTGF1.
出处
《中华肝脏病杂志》
CAS
CSCD
1999年第4期193-195,共3页
Chinese Journal of Hepatology
关键词
转化生长因子Β
病毒性肝炎
免疫组化
原位杂交
Transforming growth factor 1 Hepatitis, viral Immunohistochemistry in situhybridhation