正丁酸钠逆转人肝癌细胞恶性表型的动态观察

Observision on phenotypic reversion of human hepatocarcinoma cell line induced by sodium butyrate

目的探讨正丁酸钠对人肝癌细胞恶性表型的逆转作用。方法以0．2mmol／L的正丁酸钠（sodiumbutyrate，SB）作用于培养的人肝癌细胞GHC－3，动态观察癌细胞生长、形态、ConA凝集性、琼脂集落形成率、染色体数目、DNA合成、DNA含量等表型的变化。结果SB作用7天，人肝癌细胞生长抑制；SB作用15天ConA凝集现象减弱；作用30天后细胞形态由多角型转变为棱形，细胞ConA凝集现象基本消失；经SB作用15天、30天、45天的细胞球脂集落形成率由对照组的4．54％下降为2．53％、1.45％、1．31％；SB作用30天、45天细胞3小时3H－TdR掺入率由2330．24士10．31cpm／105cells下降为1372．5123．41cpm／105cells和1136．23土17．23cpm／105cells，细胞DNA含量由198．511．18μg/107cells下降为134．78土0．53μg/107cells和104．78土0．53μg/107cells；均有显著差异（P＜0．01）。染色体主流范围变窄，二倍体比例逐渐升高而亚三倍体比例逐渐下降，三倍体消失。结论人肝癌细胞在正了酸钠持续作用下，某些恶性表型可发生进行性逆转。

To observe the phenotypic changes of human hepatocarcinoma cell line induced by sodium butyrate. Methods GHC-3 cell line was cultured in vitro in sodiurn butyrate in a concentration of 0. 2mmol/L to observe the changes of cell morphology,growth rate, ConA aggluti nalbility, colony-forming rate, chromosome number, 3H-TdR incorporation rate, DNA concentration. Results Morphology changed from polygonal to spindle-shaped,proliferation rate was inhibited sig nificantly,ConA agglutinability disappeared. Colony-forming rate decreased gradually, the major range of chromosome turned from 52 - 59 to 44 - 45, the pencentages of diploid and hypodiploid increased gradually; the 3H-TdR incorporation rate and DNA concentration decreased gradually. Conclusion the malignant phenotypes of the human hepatocarcinoma cell line, GHC-3,can be reversed by sodium butyrate.