大鼠β-防御素rBD-1cDNA克隆

Cloning of Rat β-defensin rBD-1 cDNA

为开展β－防御素基因表达调控分子机制及其转基因防治粘膜感染的研究，作者克隆了大鼠β－防御素ｒＢＤ－１ ｃＤＮＡ。从大鼠肾脏组织提取ＲＮＡ，采用ＲＴ－ＰＣＲ技术扩增大鼠β－防御素ｒＢＤ－１ｃＤＮＡ，并重组到ｐＧＥＭ－Ｔ Ｅａｓｙ 载体，经限制性内切酶谱分析和ＤＮＡ序列测定，证实所克隆的ｃＤＮＡ片段为大鼠β－防御素ｒＢＤ－１ ｃＤＮＡ。

This is a study aimed at the molecular mechanisms of β defensins gene expression and it's gene transfer experiments for preventing mucosal infection. A rat rBD 1 cDNA was cloned. The total RNA was isolated from rat kidney. The cDNA fragment was amplified by RT PCR with specific primers. The purified RT PCR product was cloned in pGEM T Easy vector. The results of restriction endonuclease pattern analysis of the recombinant plasmid, DNA sequencing, and aligning of the putative amino acid sequence with hBD 1 and mBD 1 demonstrated that rat β defensin rBD 1 gene was cloned successfully.