肝细胞癌患者GB病毒C感染状况及其相关性研究

GB Virus-C Infection in Chinese Patients with Hepatocellular Carcinoma

为了解ＧＢ病毒Ｃ（ＧＢＶ－Ｃ）感染与肝细胞癌（下称肝癌）之间可能的相关性，采用一步法逆转录聚合酶链反应（ＲＴ－ＰＣＲ）对１２４ 例肝癌患者进行了ＧＢＶ－ＣＲＮＡ 检测。结果：ＧＢＶ－ＣＲＮＡ 总检出率高达２６．６％ （３３／１２４），３３ 例ＧＢＶ－ＣＲＮＡ 检出阳性者中，９０．９％ 合并有ＨＢＶ、ＨＣＶ感染；３３ 例非Ｂ非Ｃ型肝癌中，ＧＢＶ－ＣＲＮＡ检出率为９．１％ （３／３３）。３３ 例ＧＢＶ－Ｃ感染阳性肝癌患者中，９０．０％ （３０／３３）有既往输血史，高于ＧＢＶ－Ｃ感染阴性组（４８／９１）。上述结果提示，我国肝癌患者中ＧＢＶ－Ｃ感染的检出率高于美、欧、日等地同类患者的检出率，其在肝细胞癌及输血后肝炎发生中的病因学意义值得注意。

Hepatitis B and C viruses (HBV, HCV) are closely related with hepatocellular carcinoma(HCC). The association between the new discovered GB virus C (GBV C) and HCC has not yet been known. In this study, 124 HCC patients were detected for the prevalence of GBV C RNA by the one step nested reverse transcription polymerase chain reaction (RT PCR) and followed by hybridization using GBV C probes located at 3′ untranslated region (3′ UTR) from its reported genomes. The results showed that 33 of 124 (26.6%) HCC cases were GBV C RNA positive, including 12 cases positive HBsAg and anti HCV, and 3 for cases negative HBsAg and anti HCV. The clinical background of the patients with HBsAg and/or anti HCV who were also positive for GBV C RNA did not differ from the background of those who were negative for GBV C RNA except the ratio of blood transfusion history. In conclusion, GBV C has a high prevalence in Chinese HCC patients. Even though no sufficient data supports the causality of GBV C on hepatocarcinogenesis, further researches aimed at whether GBV C infection aggravates the incidence of HCC are warranted.