NKC、IL-2、IL-6和TNFα在海洛因依赖者戒毒过程中的变化及意义

Changes and Significance of Natural Killer Cell, IL-2,IL-6 and TNFα of Heroin Addicts after Detoxification

为了解在强制戒毒过程中，外周血自然杀伤细胞（ＮＫＣ）活性及单个核细胞分泌的ＩＬ－２ 活性变化，以及外周血清中ＩＬ－６ 及肿瘤坏死因子α（ＴＮＦα）水平的变化，对５１ 例吸毒成瘾者采用生物活性测定法（ＭＴＴ）测定了ＮＫＣ活性及ＩＬ－２活性，用ＥＬＩＳＡ法测定了外周血清中ＩＬ－６ 及ＴＮＦα水平。结果：海洛因依赖者ＮＫＣ３０．１１±５．２％ ；ＩＬ－２ ８．０６±１．６６ＩＵ／ｍ ｌ；ＩＬ－６ ６１．１７±１２．０７ｐｇ／ｍ ｌ；ＴＮＦα９１．８３±１９．１９ｐｇ／ｍ ｌ。正常对照上述各项指标分别为４４．８９±４．７５％ ，１５．９１±３．８３ＩＵ／ｍ ｌ，２２．１８±９．３１ｐｇ／ｍ ｌ，３０．５５±１１．９４ｐｇ／ｍ ｌ。经强制戒毒后ＮＫＣ和ＩＬ－２ 活性及ＩＬ－６ 和ＴＮＦα水平逐渐恢复正常，与戒毒时间呈明显相关。

In order to find out the activity of NKC and production of IL 2 in the peripheral blood mononuclear cells and the level of IL 6 and TNFα in the peripheral blood serum, we investigated 51 herion addicts who were in the course of detoxification. MTT colorimetric assay and ELISA assay methods were adopted. The results showed that, in the herion addicts, NK activity was 30.11±5.2%, IL 2 activity was 8.06±1.66 IU/ml, IL 6 level was 61.17±12.07 pg/ml, and TNFα level was 91.83±19.19 pg/ml, whereas in the healthy controls, the results were 44.89±4.75%, 15.91±3.83 IU/ml, 22.18±9.31 pg/ml and 30.55±11.94 pg/ml respectively. When the herion addicts were subjected to detoxication, their NKC and IL 2 activities, IL 6 and TNFα levels gradually restored to normal, and were correlated with the time of their detoxification (NKC: r=0.626 , P<0.001 ; IL 2: r=0.684,P<0.001 ; IL 6: r=-0.791,P<0.001; TNFα: r=-0.703,P<0.001) .