摘要
为研究萘丁美酮的相对生物利用度,建立了用反相高效液相色谱法测定血浆中萘丁美酮的活性代谢物6-甲氧基-2-萘乙酸(MNA)浓度的方法。实验结果表明,以萘普生作内标,甲醇∶0.02m ol/L醋酸盐缓冲液(pH3.0)为74∶26 作流动相,用YWG-C18 柱有良好的分离效果;血浆样品在pH3.0醋酸盐缓冲液存在下,用二氯甲烷萃取,回收率高,在270nm 波长下检测杂质峰少。该法标准曲线在0.5~64m g/L范围内有良好线性,最低检测浓度为0.02m g/L,萃取回收率为88% ~94% ,方法回收率为96% ~102% ,日内相对标准差小于3.5% ,日间相对标准差小于5% 。具有快速简便,灵敏准确等特点。
This paper reports a sensitive and rapid method for determining 6 methoxy 2 naphthylacetic acid(MNA), a major metabolite of nabumetone in human plasma using naproxen as the internal standard. High performance liquid chromatograph model 680(Waters, USA) with a variable wavelength UV detector and reversed phase YWG C 18 column(10μm, 250×4.6mm) was used. After the addition of acetate buffer(pH3.0), the plasma sample was extracted with methylene chloride. The mobile phase of methanol pH3.0, 0.02 mol/L acetate buffer(74∶26) was pumped at 1.0 ml/min through the column. The detector at 0.01AUFS was set at 270nm. The retention times for MNA and naproxen were 3.98min and 4.73 min respectively. Standard curve was linear in the concentration range of 0.5 to 64 mg/L. The detection limit in serum was 0 02 mg/L. Extraction recovery was 88% 94%; method recovery 96% 102%; withinday RSD less than 3.5%; inter day RSD less than 5%.
出处
《华西医科大学学报》
CSCD
1999年第4期452-454,共3页
Journal of West China University of Medical Sciences
关键词
萘丁美酮
代谢物
血药浓度
HPLL
Nabumetone 6-methoxy 2-naphthylacetic acid Plasma drug concentration Reversed phase HPLC