高效液相色谱法同时测定血浆中的肾上腺素和去甲肾上腺素

Simultaneous Determination of Norepinephrine and Epinephrime in Plasma by Using High Performance Liquid Chromatograph

为同时测定人血浆中肾上腺素和去甲肾上腺素浓度，建立了高效液相色谱－电化学检测方法，采用ＹＷＧ－Ｃ１８分析柱，３，４－二羟基苄胺作内标，以０．０５ｍ ｏｌ／Ｌ醋酸盐缓冲液（ｐＨ３．０）－甲醇－０．０５ｍ ｏｌ／ＬＥＤＴＡ－Ｎａ２（７８∶２０∶２）为流动相，每１Ｌ流动相含ＳＤＳ２００ｍ ｇ，流速为１．０ ｍ ｌ／ｍ ｉｎ。去甲肾上腺素和肾上腺素及内标的保留时间分别为７．３、８．３ 和１０．４ 分钟，线性范围均为３１．２５～４０００ｎｇ／Ｌ（ＮＥ：ｒ＝ ０．９９９９，Ｅ：ｒ＝ ０．９９９６）。去甲肾上腺素和肾上腺素日内ＲＳＤ分别低于１．８７％ 和２．０７％ ，日间ＲＳＤ分别小于８．９２％ 和９．６５％ ，平均萃取回收率分别为９３．５％ 和９１．３％ 。提示本方法快速简便，灵敏准确，适用于嗜铬细胞瘤的鉴别诊断和临床研究。

A sensitive and rapid method was reported for the determination of nerepinephrine(NE) and epinephrine(E) in plasma by using high performance liquid chromatography with an electrochemical detector(HPLC EC) and YWG C 18 column(5u).3,4 Dihydroxybenzylamine(DHBA) was used as an internal standard. The mobile phase of 0.05mol/L actate buffer(pH3.0) methanol 0.05mol/L EDTA Na 2 (78∶20∶2) with of 200mg/L SDS solution was pumped through the column with a flow rate of 1.0ml/min. The lengths of retention time for NE, E and DHBA(IS) was 7.3 min, 8.3 min and 10.4min, respectively. The standard curve was linear within the concentration range of 31.25 to 4000ng/L for NE and E (NE: r =0.9999 E: r =0.9996), The within day RSD(%) were less than 1.87% for NE and less than 2.07% for E, inter day RSD(%) were less than 8.92% for NE and less than 9.65% for E respectively. The average extraction recovery(%) for NE and E were 93.5%±5.50% and 91.3%±1.77% respectively. This method has been applied to the diagnosis of pheochromocytoma and clinical research.