miR-199a在宫颈癌及宫颈上皮内瘤样病变中的表达及意义

Clinical Significance of miR-199a Expression in Cervical Cancer and Cervical Intraepithelial Neoplasia

目的探讨miR-199a在宫颈癌及宫颈上皮内瘤样病变(CIN)中的表达情况及与宫颈癌各种临床病理特征的关系。方法采用茎环Realtime RT-PCR方法检测48例宫颈癌、12例CIN及正常宫颈组织中miR-199a-3p和miR-199a-5p的表达,分析miR-199a-3p和miR-199a-5p表达与宫颈癌常见的临床病理特征的关系。结果用茎环Realtime RT-PCR方法检测miR-199a-3p和miR-199a-5p表达的敏感性和特异性良好;miR-199a-3p和miR-199a-5p在宫颈癌及CIN组织中的表达低于非肿瘤组织,差异性显著(P<0.05),miR-199a-5p/miR-199a-3p表达比值未见明显差异(P=0.219)。其中miR-199a-5p的表达与宫颈癌的组织分化程度存在一定相关性。小细胞型(Ⅲ级)宫颈癌的miR-199a-5p表达低于其他组织分化类型的标本(P=0.054))。miR-199a-3p和miR-199a-5p的表达与年龄、肿块大小、大体类型、病理类型及FIGO分期未见显著相关性(P>0.05)。结论 miR-199a-3p和miR-199a-5p在宫颈癌及CIN组织中的表达显著低于正常宫颈组织。miR-199a-5p的表达与肿瘤的分化程度存在一定相关性。miR-199a在宫颈癌组织中的异常表达,可能在宫颈癌的发生和发展过程中发挥重要作用,有望成为宫颈癌新的治疗靶点。

Objective To explore the expression of miR - 199a in cervical cancer and cervical intraepithelial neoplasia （CIN） and study its association with clinicopathologie features of cervical cancer. Methods The expression levels of miR - 199a - 3p and miR - 199a- 5p in 48 cervical cancer, 12 CIN and 20 non- tumor tissue specimens were examined by stem- loop real- time RT- PCR. The correlations between the expression levels of miR - 199a -3p and miR - 199a -5p and related elinicopathologic features of cervical cancer （age, tumor size, general type, pathological type, tissue differentiation, FIGO stage） were further analyzed. Results The stem - loop RT reahime - PCR was sensitive and specific enough to detect miR - 199a - 3p and miR - 199a - 5p. Expression of miR - 199a - 5p and miR - 199a - 3p in cervical cancer and CIN tissues was obviously lower than that in the non - tumor tissue specimens（ P 〈 0.05 ）. Howev- er, there was no significant difference in ratio of the miR - 199a - 5p / miR - 199a - 3p expression （ P = O. 219）. Expression of miR - 199a -5p was associated with tissue differentiation in cervical cancer. The expression of miR -199a- 5p in low differential cervical canc- er was lower than that in middle - and high - differential tissues（ P = O. 054）. No significant association was found between miR - 199a - 5p and miR - 199a - 3p levels and other clinicopathologic features of cervical cancer, such as age, size of tumor, general type, pathologi- cal type, FIGO stage. Conclusion Expression levels of miR - 199a - 3p and miR - 199a - 5p in cervical cancer and CIN tissues are sig- nificantly lower than in normal cervical tissues. Low - regulated expression of miR - 199a - 5p was associated with tissue differentiation of cervical cancer. These data indicated that miR - 199a may play an important role in the development of cervical cancer and be a novel potential therapeutic target for cervical cancer.