阿仑膦酸钠对破骨细胞分化中c-fos基因表达的影响

Effect of Alendronate Sodium on c-fos Gene in Osteoclast Differentiation

目的探讨阿仑膦酸钠对破骨细胞分化中c-fos基因的作用机制。方法将不同浓度的阿仑膦酸钠作用于小鼠破骨细胞,采用Western blot检测小鼠破骨细胞分化中c-fos基因的表达情况。结果阿仑膦酸钠组TRAP阳性破骨细胞数目和c-fos基因的表达显著低于空白对照组;且加入10-4mol/L、10-6mol/L、10-8mol/L浓度阿仑膦酸钠后,c-fos表达受抑制程度依次增强;而在10-8mol/L、10-10mol/L、10-12mol/L浓度中,c-fos表达受抑制程度依次减弱。结论阿仑膦酸钠可使破骨细胞分化中的c-fos基因受到抑制,其抑制程度与阿仑膦酸钠的浓度有关,10-8mol/L浓度时抑制作用最强。

Objective To study the probably mechanism of alendronate sodium to c - fos gene in osteoclast differentiation. Methods Different concentrations of alendronate sodium had effect on mice osteoclast, c - fos expression in mouse osteoelast differentiation was detedcted using Western blot. Results The number of TRAP＊ osteoclasts and the expression of c - los gene from alendronate group were significantly lower than those in control group （ P 〈 0.05） , and the expression of c - los was enhanced with the concentration increase of alendronate in 10^-4 mol/L, 10^-6 mol/L, 10^-8 mol/L, but the expression of c -los was decreased with the concentration increase of alendronate in 10^-8 mol/L, 10^-10mol/L, 10^-12mol/L. Conclusion Alendronate sodium can make osteoelast differentiation of c -los gene robe restrained. Its inhibition degree was associated with alendronate sodium concentration. The strongest inhibition effect on c - los expression was at concentration of 10^-8 mol/L.