肝细胞性肝癌组织中CCR7和CXCR7的表达及其与淋巴转移的关系

Expression of CCR7 protein and CXCR7 protein in hepatocellular carcinoma and the relationship with lymphatic metastasis

目的:探讨CCR7和CXCR7在肝细胞性肝癌中的表达及其与淋巴转移的关系。方法:选取2007年1月—2009年12月在山东省肿瘤医院经外科手术治疗且临床资料完整的肝细胞性肝癌病例48例,其中男30例,女18例;肝门淋巴结转移者16例,男10例,女6例。采用免疫组织化学(MaxVision)法检测48例肝细胞性肝癌病例肝癌组织、癌旁组织、癌以远组织和肝门淋巴结中CCR7、CXCR7的表达。结果:CCR7在肝细胞性肝癌组织、癌旁组织和肝门淋巴结中的阳性率分别为75.00%(36/48)、62.50%(30/48)和64.58%(31/48),在癌以远组织中的阳性率为27.08%(13/48);淋巴结转移组的肝癌组织中CCR7的表达明显高于非淋巴转移组,两者比较差异有统计学意义(P<0.05)。CXCR7在肝癌组织、癌旁组织和肝门淋巴结中的阳性率分别为:87.50%(42/48)、64.58%(31/48)和79.17%(38/48),在癌以远组织中的阳性率为27.08%(13/48);淋巴结转移组癌旁组织中CXCR7的表达明显高于非淋巴转移组,两组比较差异有统计学意义(P<0.05)。结论:CCR7和CXCR7在肝细胞性肝癌组织中及淋巴结组织中表达增高,CXCR7和CCR7的高表达与肝细胞性肝癌的淋巴转移相关。

Objective：To investigate the expression of CCR7 protein and CXCR7 protein in hepatocellular carcinoma and the relationship with lymphatic metastasis.Methods：The tissue samples including hepatocellular carcinoma,the adjacent tumour tissues,the far tumour tissues,were obtained from 48 patients with clinical data and consent Integrity.The expression of CCR7 protein andCXCR7 protein were detected by immunohistochemistry with metastasis of porta hepatis lymph nodes in hepatocellular carcinoma.Results：The positive rates of CCR7 protein in hepatocellular carcinoma、in adjacent tumour tissues and in far tumour tissues were respectively 75.00%（36/48）,62.50%（30/48）and 27.08%（13/48）.The positive rates of CCR7 protein in porta hepatis lymph nodes was 64.58%（31/48）,the positive rates of CCR7 protein in tumour tissues with porta hepatis lymph metastasis was 87.50%（14/16）,the positive rates of CCR7 protein in tumour tissues with no porta hepatis lymph metastasis was 53.13%（17/32）,there were significant differences in the 48 case of hepatocellular carcinoma.The positive rates of CXCR7 protein in hepatocellular carcinoma、in adjacent tumour tissues and in far tumour tissues were respectively 87.50%（42/48）、64.58%（31/48）and 27.08%（13/48）.The positive rates of CXCR7 protein in porta hepatis lymph nodes was 79.17%（38/48）.Conclusion：The expression of CCR7 protein and CXCR7 protein in hepatocellular carcinoma is higher than the far tumour tissues;the high level of CXCR7 protein and CCR7 protein in hepatocellular carcinoma is related with the lymphatic metastasis.