抑制乙肝病毒X蛋白表达对肝癌细胞上皮一间质转化和凋亡的影响

Effect of Inhibiting of HBx expression on epithelial-mesenchymai transition and apoptosis of liver cancercells

目的探讨乙肝病毒x蛋白（HBx）对肝癌细胞转移能力的影响及其机制。方法采用siRNA干扰技术抑制MHCC97H细胞的HBx表达。通过Transwell小室和裸鼠肺转移模型实验比较HBx抑制前后MHCC97H细胞转移能力的变化。应用Western blotting技术检测肝癌细胞上皮间质转化（EMT）和凋亡相关基因的表达变化。结果将Hgx-siRNA导入MHCC97H细胞可以抑制HBx的表达及其转移能力，可下调EMT相关分子Twist和N—cadherin的表达，并上调E—cad—herin表达而抑制EMT，同时还可通过Twist—P53途径诱发细胞的凋亡。结论抑制HBx表达可以通过改变上皮一间质转化和诱发凋亡而减弱高侵袭肝癌细胞MHCC7H的转移能力。

Objective To investigate the effects and possible mechanism of action of inhibiting hepatitis B virus X protein （HBx） expression on liver cancer metastasis. Methods The suppression of HBx expression in MHCC97H cells was performed by siRNA interference technique, and the effects of HBx suppression on the metastasis of MHCC97H cells were detected by Matrigel invasion assays and in a lung-metastasis mouse model. The expression levels of related epithelial-mesenchymal transi- tion （EMT） and apoptosis proteins were examined by Western blotting. Results Introduction of HBx-siRNA into MHCC97H cells inhibited the expression of HBx and the ability to metastasize, downregulated the expression of Twist and N-cadherin, and upregulated E-cadherin expression. These changes resulted in inhibiting EMT of MHCC97H cells. Meanwhile, apoptosis involved in the Twist- P53 pathway was also found. Concluslbns Inhibiting expression of HBx can decrease the metastatic a- bility of MHCC97H ceils by changing EMT and inducing apoptosis.