HPLC法测定利胆消石片中大黄素、大黄酚的含量

Determination of Emodin and Chrysophanoc in Lidan Xiaoshi Pian by HPLC

目的采用高效液相色谱法测定利胆消石片中大黄素及大黄酚的总量。方法采用AgilaVenusil MP-C18色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈-甲醇-0.1%磷酸溶液(42:23:35);流速:1.0 mL min-1;柱温:35℃;检测波长:376 nm。结果大黄素、大黄酚的线性范围分别为18.26～456.5ng(r2=1.000 0)、40.56～1 014ng(r2=1.000 0),平均回收率(n=6)分别为99.3%、100.8%。结论该方法准确、简便,具有良好的重复性和稳定性,适合于利胆消石片中大黄素、大黄酚的含量测定。

Objective To develop an HPLC method for determine the content of emodin and chrysophanoc in Lidan Xiaoshi Pian. Methods This analysis was performed on an Agila Venusil MP-C18 column （ 250 mm×4.6 mm, 5 μm ） ; The mobile phase was acetonitrile-methanol-0.1% phosphoeic acid solution; The flow rate was 1.0 mL·min^-1 ; the column was maintained at 35 ℃, and the detection wavelength was set at 376nm. Results The linear ranges were18.26-456.5 ng （ r^2=1.000 0 ） for emodin, 40.56-1 014ng （ r^2=l.000 0 ） for chrysophanoc; The average recoveries （n=6） of 4 components were 99.3%, 100.8%.Conclusion The developed method is simple, rapid and accurate with high repeatability and suitable for the quantitative analysis of emodin and chrysophanoc in Lidan Xiaoshi Plan.