家蝇蛆粉的临床前致畸性研究

Study on the preclinical teratogenicity of musca domestica larvae powder

目的探讨家蝇蛆粉对大鼠的致畸性,以评价其安全性。方法家蝇蛆粉按比例定量添加到普通饲料原料中混匀,干燥,做成高、中、低剂量家蝇蛆粉饲料。将性成熟的98只SD大鼠按雌雄2∶1的比例同笼交配。取64只孕鼠平均分为4组:家蝇蛆粉饲料低剂量组;家蝇蛆粉饲料中剂量组;家蝇蛆粉饲料高剂量组;普通饲料对照组。于受孕后称量体质量增长情况。最后脱颈椎处死孕鼠,称子宫质量,并进行胎鼠检查。结果家蝇蛆粉饲料高、中、低剂量组与对照组相比,雌鼠体质量增长、子宫质量比较差异无统计学意义,无吸收胎、早死胎、晚死胎;各组间雄性与雌性胎鼠数比较差异均无统计学意义;各组间雄性与雌性胎鼠的外观、体质量、体长比较差异均无统计学意义。各组间胎鼠骨骼和胎鼠内脏比较差异均无统计学意义。结论家蝇蛆粉对SD大鼠未见致畸性。

Objective To evaluate the teratogenicity on mice of powder extract from Musca domestica larvae powder（MDLP） and the safety of it.Methods MDLP was added into animal feeds,then we prepared high dose of MDLP animal feeds,middle dose of MDLP animal feeds,and low dose of MDLP animal feeds.In each cage,2 female eugamic rats and 1 male eugamic rat were put together for mating.64 pregnant rats were divided into the 4 groups：high dose treated group,middle dose treated group,low dose treated group,and ordinary feedstuff treated group as controls.The growth information of the weight of the above 4 group were analyzed.At last,uteruses of pregnant rats were weighed and the fetuses were examined.Results The growth information of whole weight and uteruses weight of pregnant rats,and the appearance,sex,weight,stem length,bone,internal organs of rat fetus of three different dose treated groups were not significantly different compared with those of the control one.No absorption foetus,earlier died foetus before delivery,later died foetus before delivery was found in three different dose treated groups.Conclusion There are no embryonic and teratogenic toxicity teratogenicity for MDLP on SD mice.