摘要
多基因转化是当今遗传转化研究的热点之一,在植物基因工程中利用有自我剪切功能的口蹄疫病毒片段2A和凤仙花种子的一段多肽LP4作为连接肽进行多基因融合是一种可以替代传统多基因转化方法的新手段,连接肽可以同时连接多个基因并且保证多基因的协同表达,将LP4和2A多肽杂合形成的新的多肽(LP4/2A)兼具两者优势,是一种更为有效的多基因转化策略。
Multiple genes transformation is one of the hot spots in modern genetic transformation research. Using 2A from foot and mouth virns and LP4 from Raphanus sativus seeds as linkers for multiple genes transformation is a new strategy which can be substitute traditional methods in plant genetic engineering. The linker peptide can be used for connecting multiple genes and genes are expressed coordinately. The hybrid peptide LP4/2A has the both advantages of LP4 and 2A which is a more effective peptide for multiple genes transformation.
出处
《生物技术进展》
2011年第1期21-25,共5页
Current Biotechnology
基金
国家自然科学基金项目(30771383和30970231)资助
关键词
口蹄疫病毒2A片段
连接肽LP4
多基因转化
2A fragment of foot-and-mouth disease virus（FMDV）
linker peptide LP4
multiple gene transformation
