依达拉奉对大鼠局灶性脑缺血后神经元凋亡的影响

目的探讨依达拉奉对大鼠局灶性脑缺血后神经细胞的保护作用。方法采用线栓法制作大鼠大脑中动脉闭塞模型(MCAO),将动物随机分为假手术组、生理盐水组和依达拉奉组,采用比色法测定脑组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力,免疫组化法检测caspase-3的表达水平,原位末端标记(TUNEL)法检测凋亡水平。结果依达拉奉治疗组与生理盐水组相比,脑组织中MDA含量显著降低(P<0.05),SOD活力显著升高(P<0.05);同时依达拉奉治疗组的脑神经元caspase-3的表达水平及细胞凋亡水平比生理盐水组显著减少(P<0.05)。结论依达拉奉可有效提高大鼠局灶性脑缺血时脑组织中SOD活力,降低MDA水平,并且有效抑制caspase-3表达,对抗神经细胞凋亡。

Objective To study the protective effects of Edaravone on rats after focal cerebral ischemia. Methods 36 male Wistar rats were randomly divided into three groups：the sham group, the NS control group and Edaravone intervention group. The model of global cerebral ischemia was induced by occlusion of the right middle cerebral artery. The Edaravone groups were given intraperitoneal injection of Edaravone（3 mg/kg）. Using the colorimetry, we analyzed the quantity of maleic dialdehyde（MDA）and enzyme activity of super oxide dismutase（SOD）of the brain tissues in all groups. The expression of caspase-3 and the apoptosis in neural cells were confirmed respectively by immunohistochemistry（TUNEL）. Results Compared with the NS group and the sham group,the quantity of MDA declined greatly（P〈0.05）, while the activity of SOD in cerebral neuron increased markedly in the Edaravone intervention group（P〈0.05）.The number of apoptotic neuron in the Edaravone intervention group was fewer than the NS group. Conclusion Edaravone can inhibit apoptosis by upgrading the activity of SOD,downgrading the quantity of MDA probably and inhibit the express of caspase-3. It will be a good medicine in cerebral ischemia.