外周血T淋巴细胞、高敏C反应蛋白与急性病毒性心肌炎的相关性研究

Study of peripheral blood T lymphocytes,high-sensibility C reactive protein associated with acute viral myocarditis

目的探讨外周血T淋巴细胞、高敏C反应蛋白与急性病毒性心肌炎的关系。方法以怀柔区第一医院院2008年1月-2010年12月确诊为病毒性心肌炎的43例患者为研究对象(观察组),采用流式细胞术对外周血进行CD3、CD4、CD8淋巴细胞亚群检测,酶联免疫吸附法(ELISA)测定高敏C反应蛋白(high-sensibility C re-active protein,hs-CRP)的系列变化值,取其峰值。对照组为正常人群30名。结果外周血T淋巴细胞亚群CD3的数量两组无差别(P>0.05)。与对照组相比,观察组外周血T淋巴细胞亚群CD4的数量低于对照组,而CD8的数量明显高于对照组(P<0.05)。hs-CRP峰值与CD4/CD8比值呈负相关(r=-0.693,P<0.05)。结论急性病毒性心肌炎发生发展与自身细胞免疫、炎症因子相关。

Objective To analyze the relationship between peripheral T lymphocytes,high-sensibility C reactive protein（hs-CRP） and acute viral myocarditis（AVMC）.Methods Tottally 43 cases of AVMC diagnosed during January 2008 to December 2010 were study group.Peripheral blood CD3,CD4,CD8 lymphocyte subsets were detected by flow cytometry.The peak of CRP was detected by the enzyme linked immunosorbent assay（ELISA）.30 cases of normal were control group.Results The number of CD3 T lymphocyte subsets in peripheral blood was no difference（P〉0.05） in groups.Compared with the control group,the number of peripheral blood CD4 T lymphocyte were significantly decreased and the number of CD8 T lymphocyte was significantly increased（P〈0.05）.The peak of hs-CRP was negatively correlated with the CD4/CD8 ratio（r=-0.691,P〈0.05）.Conclusions Cellular immune suppression,inflammatory relevant with the development of AVMC