HLA-DRB1等位基因多态性对乙型肝炎患者预后的影响

HLA-DRB 1 gene polymorphism and its relation with hepatitis B infection in Henan Han nationality

目的研究HLA-DRBl等位基因多态性与河南汉族乙型肝炎预后的相关性。方法以河南汉族慢性乙型肝炎、乙型肝炎病毒携带者、原发性肝癌、健康组对照组为研究对象,应用聚合酶链反应-序列特异性引物技术(PCR-SSP)对研究对象的HLA-DRB1等位基因多态性进行分析比较。结果 HLA-DRB1*0701、1001、1201、1501在河南汉族慢性乙型肝炎组分布频率显著高于健康对照组(P<0.05),而HLA-DRB1*1301(2)显著低于健康对照组(P<0.05);HLA-DRB1*0701、1001、1201、1501在河南汉族慢性乙型肝炎和乙型肝炎病毒携带者组分布频率差异无统计学意义(P>0.05);HLA-DRB1*1501在原发性肝癌组分布频率显著高于健康对照组(P<0.05),RR=13.72。结论等位基因HLA-DRB1*0701、1001、1201、1501是河南汉族慢性乙型肝炎和乙型肝炎病毒携带者的易感基因,HLA-DRB1*1501是原发性肝癌的易感基因,HLA-DRB1*1301(2)是河南汉族乙型肝炎的保护性基因。

Objective To study HLA-DRB 1 gene polymorphism and its relation with hepatitis B infecton in Henan Han nationality.Methods HLA-DRBl genotyping was conducted in patients of chronic hepatitis B,asymtomatic carrier and hepatocarcinoma patients as well as health controls by using polymerase chain reaction-sequence specific primer method.Results Compared with health controls,the allele frequency of HLA-DRBl＊ 0701、1001、1201、1501 were markedly higher（P〈0.05） in chronic hepatitis B patients and asymtomatic carriers,but the allele frequency of HLA-DRBl＊1301（2） was lower than the controls,there were no significant difference of the allele frequency of HLA-DRB1 ＊0701、1001、1201、1501in chronic hepatitis B patients and asymptomatic carriers（P〉0.05）,the allele frequency of HLA-DRB1 ＊1501 was higher in hepatocarcinoma patients than the controls（P〈0.05）,RR=13.72.Conclusions HIA-DRB1＊0701、 1001、 1201、 1501 are closely related with susceptibility to chronic hepatitis B,asymtomatic carriers,especially the HIA-DRB1＊1501 is closely related with resistance to hepatocarcinoma and HLA-DRBl 1301（2） alleles are protective genes for hepatitis B patients in Henan Han nationality