酶解骨粉对大鼠钙吸收及骨密度的影响

Effects of enzymatic bone powder on calcium absorption and bone density in rats

目的观察酶解牛骨粉、超微酶解牛骨粉、酶解鱼骨粉的补钙效果。方法 90只SPF级4周龄雌性SD大鼠随机分为9组:酶解牛骨粉、超微酶解牛骨粉、酶解鱼骨粉、碳酸钙低、高剂量组以及低钙对照组,喂养13周。于试验第3周末做代谢试验,测量钙吸收率和骨密度。结果酶解骨粉钙表观吸收率34.0%～67.93%,均明显低于低钙对照组(87.1%);碳酸钙高剂量组钙表观吸收率高于相应剂量试验组(P<0.05),其他各组无统计学差异(P>0.05)。酶解骨粉各组大鼠股骨中点、远心端和近心端骨密度均明显高于低钙对照组(P<0.05);酶解牛骨粉、超微酶解牛骨粉和酶解鱼骨粉组一点或两点骨密度高于相应剂量碳酸钙组(P<0.05)。结论酶解牛骨粉、超微酶解牛骨粉、酶解鱼骨粉均可有效改善大鼠的骨密度,其中超微酶解牛骨粉和酶解鱼骨粉增加大鼠骨密度效果好于碳酸钙,但钙表观吸收率却较碳酸钙低。

Objective To identify the influence of enzymatic cattle bone,enzymatic fish bone and ultrafine-enzymatic cattle bone on apparent calcium absorption rate and bone density in rats.Methods Female SD rats were randomly divided into 9 groups with 10 rats in each group,and were fed with low-and high-enzymatic cattle bone diet,low-and high-enzymatic fish bone diet,low-and high-ultrafine-enzymatic cattle bone diet,low-and high-calcium carbonate diet and low calcium control diet for 13 weeks.The calcium content of the low and high experimental groups was 500mg/100g and 1000mg/100g,and the calcium content of the control group was 100mg/100g.3-day calcium metabolic experiment was conducted by the end of the 3rd week.Bone density was assayed by the end of the 13th week.Results The apparent calcium absorption rate was related to the calcium content in diet in a range of 60.69%-65.84% in the low dose groups and 34.00%-44.06% in the high dose groups.The apparent calcium absorption rate in experimental groups was obviously lower than that in the low calcium control group（P0.05）.Bone density of rats in enzymatic cattle bone,enzymatic fish bone and ultrafine-enzymatic cattle bone groups were obviously higher than that in calcium carbonate group at the same calcium content in diet（P0.05）.Conclusions According to the results of calcium apparent absorption rate and bone density,enzymatic cattle bone,enzymatic fish bone,ultrafine-enzymatic cattle bone,and calcium carbonate could be used as sources of calcium.