大豆异黄酮对72h睡眠剥夺雌性小鼠认知功能的影响

Effect of soybean isoflavones on the cognitive performance of mice deprived of sleeping for 72h

目的研究大豆异黄酮(SIF)对72h睡眠剥夺雌性小鼠认知功能的影响,并探讨其作用机制。方法将40只雌性8周龄清洁健康昆明小鼠分为正常对照组、睡眠剥夺对照组(SDC)、大豆异黄酮低、中、高剂量组(SIF L、M、H)。SIF L、SIF M和SIF H组分别给予20、40和80mg/kg BW的大豆异黄酮进行灌胃,正常对照组和睡眠剥夺对照组灌胃等体积生理盐水,连续30天。采用单平台水环境法(SPM)制作小鼠72h睡眠剥夺模型。在睡眠剥夺72h后,利用Y-型迷宫试验确定各组小鼠的认知能力,同时测定小鼠大脑组织抗氧化指标(CAT、MDA、T-AOC)和神经递质(NO、Glu、Ach)。结果与正常对照组比较,72h的睡眠剥夺降低了雌性小鼠的认知能力、大脑CAT酶活力、T-AOC水平和Ach水平,增加了大脑NO、MDA和Glu含量。与睡眠剥夺对照组比较,SIF改善了72h睡眠剥夺雌性小鼠的认知能力,提高了大脑CAT酶活力、T-AOC水平和Ach水平,降低了大脑NO、MDA和Glu含量。结论大豆异黄酮能够改善72h睡眠剥夺雌性小鼠的认知功能,这可能与其提高了大脑抗氧化能力、降低了自由基对大脑神经损害并调节了大脑神经递质有关。

Objective To study the effect and mechanism of Soybean Isoflavones（SIF） on the cognitive performance of female mice after sleep deprivation for 72h.Method Forty female mice were randomly divided into 5 groups：the control,sleep deprivation control（SDC）,and three SIF groups（SIF L,SIF M and SIF H）.SIF groups were administered by intra-gastric injection of SIF 20,40 and 80mg/kg bw respectively for 30 days.The control and SDC groups were administered with equal volume of normal saline.The 72h SD mice model was induced by the single platform technique.The cognitive ability of mice was tested by Y-maze.Antioxidant indicators（CAT,MDA and T-AOC） and neurotransmitters（NO,Glu and Ach） in cerebra were measured.Result Compared with the control group,the cognitive abilities,CAT activities,T-AOC and Ach levels were decreased and NO,MDA and GLu contents were increased in mice after sleep deprivation for 72h.Compared with the SDC group,the cognitive abilities were improved and Ach contents were increased in SIF M and SIF H groups,T-AOC levels were improved in three SIF groups.CAT activities,MDA,NO and Glu contents were decreased in SIF H group only.Conclusion The cognitive ability of 72h SD female mice could be improved by SIF,the mechanism of which might be involved in increasing antioxidant function,decreasing the damage of free radicals on cranial nerves and adjusting the neuron transmitter of cerebra.