一株具有免疫增强活性的海南红树林真菌WC1016的分类鉴定

Classification and identification of mangrove fungi strain WC1016 possessing immunity activity

目的:对从海南文昌清澜港红树林土壤样本中分离到的一株具有免疫增强活性的真菌菌株WC1016进行分类鉴定。方法:通过菌落特征、显微形态、ITS序列测定及其系统发育分析对菌株WC1016进行鉴定。结果:菌株WC1016在CA上形成典型紫色绒絮状菌落。显微观察其分生孢子梗具有拟青霉属的典型特征。ITS序列测定及系统发育分析表明菌株WC1016与淡紫拟青霉(Paecilo-myces lilacinus)菌株XLA同源性最高。结论:通过形态学观察和分子生物学分析鉴定菌株WC1016为淡紫拟青霉。首次鉴定发现一株具有免疫增强活性的淡紫拟青霉。

Objective： To classify and identify the strain WC1016 possessing immunity activity from mangrove soil of Qinlan harbor in Hainan. Methods：The strain of WC1016 was identified by culture characteristics, morphological characteristics, ITS sequence and phylogenetic analysis. Results. It was shown that the strain of WC1016 on CA medium produced lilacinus powder colony. Under microscope, its conidiophore revealed distinct structure that was unique to Paecilomyces. Further ITS sequence analysis showed the strain had the highest homology with Paecilomyces lilacinus suggesting that they were proba bly conspecific. Conclusion：The strain WC1016 is identified as Paecilomyces lilacinus and this is the first report on a strain of Paecilomyces lilacinus possessing immunity activity.