山芝麻水提液对HepG2.2.15细胞HBsAg和HBeAg的抑制作用

Inhibitory Effect of Water Extracts from Helicteres Angustifolia on HBsAg and HBeAg in HepG2.2.15 Cells

目的观察山芝麻水提液(water extracts from Helicteres angustifolia,WHA)的体外抗乙型肝炎病毒的作用。方法采用HepG2.2.15细胞模型进行体外培养,给予不同浓度山芝麻,以拉米夫定作阳性对照,作用72 h后检测上清液中HBsAg、HBeAg的分泌,观察药物对HepG2.2.15细胞分泌HBV病毒抗原的影响,同时以MTT法检测药物在体外对HepG2.2.15细胞的生长抑制作用,从而评价药物的抗HBV作用。结果山芝麻水提液对HepG2.2.15细胞的半数细胞毒浓度(TC50)为482.1 mg.L-1;对HepG2.2.15细胞分泌HBsAg的半数抑制浓度(IC50)为7.3 mg.L-1,其治疗指数(TI)为66;对HBeAg,其IC50为14.6 mg.L-1,TI为33。结论山芝麻水提液在体外有显著的抗HBV的作用,且毒性较低。

OBJECTIVE To observe the effect of water extracts from Helicteres angustifolia （WHA） on excreting HBsAg and HBeAg of HepG2.2.15 cells. METHODS The toxicity of WHA on HepG2.2.15 cells was detected by means of MTT. The inhibitory effects of WHA on HepG2.2.15 cells excreting HBsAg and HBeAg were evaluated with method of enzyme-linked immunosorbent assay（ELISA）. RESULTS The half toxic concentration （TCs0） of WHA on HepG2.2.15 cells was 482.1 mg-L-1 The half inhibitory concentration （ICs0） of WHA on HBsAg was 7.3 mg.L-1, the trealment index （TI） of WHA on HBsAg was 66, and the ICs0 of WHA on HBeAg was 14.6 mg.L-l, TI was 33. CONCLUSION WHA can significantly inhibit HBV in vitro, and is a lower toxic drug.