摘要
目的建立芍药苷的分离纯化与结构鉴定方法,有效提高芍药苷的含量。方法将赤芍用醇水溶液加热回流提取,用AB-8大孔吸附树脂、制备液相等工序进行分离纯化,通过理化常数及光谱分析鉴定化合物结构,以HPLC测定其含量。结果该法得到的芍药苷纯度为95.4%,总回收率为81.03%。结论本工艺具有高效、简便、成本低、回收率高等优点,是一种良好的芍药苷分离纯化方法。
OBJECTIVE To establish the method of separation, purifying and idenfication method of paeonifiorin, and to elevate effectually paeoniflorin content. METHODS Paeonia veitchii Lynch was extracted with ethanol-water solution, separated and purified by AB-8 macroporous resin and preparative HPLC. The structure was identified on the basis of physical and chemical characters and spectral analysis and quantitatively determined by HPLC. RESULTS Paeoniflorin with purity over 95.4%, can be prepared with the total recovery more than 81.03%. CONCLUSION High purity products can be obtained with this process which is high-performance, convenient, low cost and high recovery and is a good method of separation and purification of paeoniflorin.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2011年第7期634-637,共4页
Chinese Journal of Modern Applied Pharmacy
基金
辽宁省自然科学基金(20092015)
沈阳市科学技术计划项目(F10-205-1-28)
辽宁大学"211工程"三期建设项目
关键词
赤芍
芍药苷
分离
纯化
AB.8大孔吸附树脂
Paeonia veitchii Lynch
paeoniflorin
isolation
purification
AB-8 macroporous resin
