雪松松针中莽草酸的分离及其纯度检查

Separation of Shikimic Acid from Pine Needles of Cedrus Deodara and Its Purity Determination

目的从雪松松针中提取分离莽草酸并进行纯度检查。方法采用水提、萃取、柱层析、重结晶等步骤分离纯化莽草酸,用NMR和理化性质鉴定其结构,再采用TLC和HPLC对其纯度进行检查。结果分离得到的莽草酸纯度达95%以上,得率为0.72%。结论该法简便经济、合理可行,可用于雪松松针中莽草酸的提取分离。

OBJECTIVE To separate shikimic acid from pine needles of Cedrus deodara and determine its purity. METHODS Shikimic acid was separated by process of water extraction, solvent extraction, silica gel column chromatography, recrystallizing etc. Its structure was identified on the basis of NMR analysis and chemical evidence and its purity was determined by TLC and HPLC. RESULTS The purity of shikimic acid was more than 95% and the total yield was 0.72%. CONCLUSION The method is convenient and reasonable, it can be used to separate the shikimic acid from pine needles of Cedrus deodara.