HPLC测定混合糖电解质注射液中果糖、葡萄糖和木糖醇的含量

Determination of Fructose,Glucose and Xylitolum in Carbohydrate and Electrolyte Injection by HPLC

目的建立混合糖电解质注射液中果糖、葡萄糖和木糖醇的含量测定方法。方法色谱柱:Waters Sugar Pak I(300mm×6.5 mm);流动相:水;流速:0.5 mL.min-1;示差折光检测器。结果果糖、葡萄糖和木糖醇峰分离度良好;果糖的线性范围为20～100μg(r=0.999 9);平均回收率为99.53%,RSD=0.81%(n=9);葡萄糖的线性范围为40～200μg(r=0.999 7);平均回收率为99.75%,RSD=0.47%(n=9);木糖醇的线性范围为10～50μg(r=0.999 7);平均回收率为99.78%,RSD=1.02%(n=9)。结论本方法简便、准确,可用于混合糖电解质注射液中果糖、葡萄糖和木糖醇的含量测定。

OBJECTIVE To establish a method for determination of fructose, glucose and xylitolum in carbohydrate and electrolyte injection by HPLC. METHODS A Waters Sugar Pak I column （300 mm×6.5 mm） was used with the mobile phase of water. Flow rate was 0.5 mL.min^-1. The tests were carried out by refractive index detector. RESULTS The resolution among fructose, glucose and xylitolum were good. The linear range of fructose was 20-100 μg（r=0.999 9）, with average recovery of 99.53%, RSD=0.81%（n=9）. The linear range of glucose was 40-200 p.g （r=0.999 7）, with average recovery of 99.75%, RSD=O.47%（n=9）. The linear range of xylitolum was 10-50 μg （r=0.999 7）, with average recovery of 99.78%, RSD=l.02%（n=9）. CONCLUSION The established method is simple, sensitive and reproducible, and suitable for the quality control of multiple electrolytes and invert sugar iniection.