组胺致Beagle犬类过敏反应实验模型研究

Experimental model of histamine-induced anaphylactoid reactionon Beagle dogs

目的:通过静脉注射磷酸组胺注射液,建立类过敏反应的Beagle犬实验动物模型。方法:一次静脉注射磷酸组胺注射液,根据注射后Beagle犬的行为及血压变化,判断类过敏反应。结果:磷酸组胺注射液静脉注射可引起Beagle犬产生典型的类过敏反应,高剂量组的反应明显强于低剂量组。结论:Beagle犬静脉注射磷酸组胺注射液能够模拟临床类过敏反应的发生过程,可以作为中药注射剂类过敏反应的敏感动物模型,用于评价中药注射剂及其组成成分类过敏反应的临床前实验研究。

Objective： To establish animal model of histamine-induced anaphylactoid reactions on Beagle dogs through intravenous injection of histamine phosphate injection. Method： Anaphylactoid reactions was determined according to the changes of praxiology and blood pressure of Beagle dogs after one intravenous injection of histamine phosphate injection. Result： It showed that typical anaphylactoid reactions be caused by histamine phosphate injection on Beagle dogs, and the response of the high-dose group was more obvious than that of the low-dose group. Conclusion： Intravenous injection of histamine phosphate injection could simulate Beagle dogs to bring typical clinical anaphylactoid reaction, that could be used as a sensitive animal model to evaluate anaphylactoid reaction of traditional Chinese medicine injections （TCMIs） in the pre-clinical experiments of both TCMIs and their composition.