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脑源性神经营养因子-B型酪氨酸激酶受体通路在大鼠切口痛机制中的作用 被引量：1

Role of brain-derived neurotrophic factor-tyrosine kinase receptor B pathway in mechanisms of rat incisional pain
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摘要 目的观察鞘内注射B型酪氨酸激酶受体(TrkB)抑制剂K252a对切口痛大鼠疼痛的影响,以了解脑源性神经营养因子(BDNF)-TrkB通路在切口痛机制中的作用。方法 27只成年雄性Sprague-Dawley(SD)大鼠随机分为切口痛(I)组、K252a+切口痛(K+I)组和二甲基亚砜(DMSO)+切口痛(D+I)组。建立大鼠切口痛模型。K+I组和D+I组分别于切口痛模型制备前30 min鞘内注射K252a 10μL+磷酸盐缓冲液(PBS)10μL和0.01%DMSO 10μL+PBS 10μL,并用PBS 10μL冲管,I组接受同样的处理但鞘内不予任何药物。分别于术前(0 d)和术后1、3、5、7 d采用von Frey纤毛法检测大鼠的机械痛阈值,采用热辐射法检测大鼠热痛阈值的变化。结果 I、D+I和K+I组术后1、3 d时的机械痛阈值均显著低于同组0 d时(P值均<0.01)。术后1、3 d时,K+I组与I组、D+I组机械痛阈值的差异均有统计学意义(P值均<0.05)。I组术后1、3、5 d时及D+I组术后1、3 d时的热痛阈值均显著低于同组0 d时(P值均<0.01)。术后1 d时,K+I组与I组、D+I组热痛阈值的差异均有统计学意义(P值均<0.05)。结论鞘内注射K252a可以减轻切口痛引起的大鼠疼痛行为学改变,BDNF-TrkB通路可能参与大鼠切口痛的形成机制。 Objective To explore the role of the brain-derived neurotrophic factor（BDNF）-tyrosine kinase receptor（Trk）B pathway in the mechanisms of incisional pain by observing the effect of intrathecal administration of tyrosine kinase blocker K252a on rat incisional pain.Methods Twenty seven adult male Sprague-Dawley（SD） rats were randomly divided into three groups,namely,group I： incisional pain group（n=9）,group K＋I： intrathecal administration of K252a plus incisional pain（n=9）,and group D＋I： intrathecal administration of DMSO plus incisional pain（n=9）.Mechanical and thermal pain thresholds were examined by von Frey and radiant heat methods respectively before operation and 1,3,5,7 day after operation.Results The mechanical pain threshold decreased at day 1 and day 3 after operation in group I,group D＋I and group K＋I compared with their corresponding pre-operational basal levels（all P0.01）.The mechanical pain thresholds in group K＋I were significantly different from those of group I and group D＋I at day 1 and day 3 after operation（all P0.05）.In group I,the thermal pain thresholds were significantly decreased compared with the basal level before operation at day 1,day 3 and day 5 after operation（all P0.01）;the same was true in group D＋I at day 1 and day 3 after operation（all P0.01）.The thermal pain threshold in group K＋I was significantly different from those of group I and group D＋I（all P0.05）.Conclusion Intrathecal administration of K252a can attenuate the aggravation of pain behaviors in rats suffering the incision pain.BDNF-TrkB pathway may be involved in the mechanisms of incisional pain in rats.
作者 赵华平 韩潞潞 薛庆生 于布为
机构地区 上海交通大学医学院附属瑞金医院麻醉科
出处 《上海医学》 CAS CSCD 北大核心 2011年第6期418-420,共3页 Shanghai Medical Journal
基金 国家自然科学基金资助(30672020)
关键词 切口痛 脑源性神经营养因子 机械痛 热痛 Incisional pain Brain-derived neurotrophic factor Mechanical pain Thermal pain
分类号 R363 [医药卫生—病理学]
  • 相关文献

参考文献10

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二级参考文献12

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共引文献2

同被引文献12

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二级引证文献1

上海医学
2011年 第6期

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