加巴喷丁对七氟烷小鼠镇痛、催眠、遗忘作用的影响

Influence of Gabapentin on analgesia,hypnosis and amnesia effects of sevoflurane in mice

目的观察加巴喷丁(GBP)对吸入七氟烷(SEV)的小鼠的镇痛、催眠和遗忘作用的影响。方法采用热板法和甩尾法观察GBP对SEV小鼠痛阈(HPPT)的影响,翻正反射实验观察各实验组小鼠睡眠潜伏期、睡眠维持时间和入睡率,避暗和跳台实验观察潜伏期和错误次数。采用翻正反射法时按分层随机分组,分为GBP(G)组、SEV(S)组和GBP+SEV(GS)组,每组10只;采用甩尾法、热板法、避暗和跳台实验时按分层随机分组,分为0.9%氯化钠溶液(NS)组、G组、S组、GS组,每组10只。结果热板法实验中,GS组给药后15、25 min的HPPT值显著高于NS组、G组及S组(P值均<0.05);甩尾法实验中,GS组给药后10、15 min的HPPT值显著高于G组,5、10、15 min的HPPT值显著高于S组(P值均<0.01)。翻正反射法实验中,GS组的睡眠潜伏期较G组显著缩短(P<0.01),睡眠时间较G组及S组显著延长(P值分别<0.05、0.01),入睡率显著高于G组及S组(P值均<0.01)。跳台实验中,GS组的跳台潜伏期较G组显著缩短(P<0.05),错误次数较G组显著增多(P<0.01);避暗实验中,GS组的避暗潜伏期较S组显著缩短(P<0.05),错误次数较S组显著增多(P<0.05)。结论 GBP与SEV合用可增强小鼠的镇痛、催眠和遗忘作用。

Objective To observe the influence of Gabapentin （GBP） on analgesia, hypnosis and amnesia effects of sevoflurane （SEV） in mice. Methods The hot-plate test and hot water tail-flick test were used to observe the influence of GBP on the analgesia effect of SEV in mice, and righting reflex test was used to observe the changes of sleep latency, sleep time and sleep ratio. Step-through test and step-down test were used to observe the latency and error times. For the righting reflex test the animals were divided into GBP（G）, SEV（S）, and GBP＋ SEV（GS） groups, with 10 mice in each group. In the rest tests, animals were divided into normal saline （NS）, G, S, and GS groups, with 10 mice in each group. Results The results of hot plate test showed that the HPPT value in GS group was significantly higher than those in G and S groups at 15 and 25 min （P〈0.05）. hot water tail-flick test showed that the HPPT value in GS group was significantly higher than those in G and S groups at 5, 10, and 15 min （all P〈0.01）. Righting reflex test showed that the sleep latency in GS group was significantly shorter （all P〈0.05）, the sleep time was significantly longer （P〈0.05 or 0.01 ）, and the sleep ratio was significantly higher than those in group G and S （P〈0.01 ）. The step-through test showed that the GS group had a significantly longer latency （all P〈0.05） and significantly more error times （P〈 0.01）. Step-down test showed that the latency in GS group was significantly shorter than that in S group （P〈0.05） and the error times were significantly increased than those in S group （P〈0.05）. Conclusion GBP can enhance the analgesia, hypnosis, and amnesia effects of SEV.