Ki-67、P27在GCB及non-GCB弥漫性大B细胞淋巴瘤中的表达及意义

Expression and significance of Ki-67 and P27 in germinal centre and non-germinal centre B-like diffuse large B-cell lymphomas

目的:探讨Ki-67、P27在GCB及non-GCB型弥漫性大B细胞淋巴瘤(DLBCL)中的表达及意义。方法:收集60例DLBCL及19例淋巴结反应性增生组织,并制作其组织芯片,采用免疫组化对DLBCL进行生发中心样B细胞(germinal center B cell-like,GCB)和非生发中心样B细胞(non-GCB)免疫表型分类,同时检测Ki-67及P27蛋白的表达。结果:(1)60例DLBCL中GCB类型29例(48.3%),non-GCB类型31例(51.7%)。(2)Ki-67及P27蛋白的表达在DLBCL中GCB及non-GCB亚型中差异均有显著性意义(均P<0.05)。(3)在DLBCL中Ki-67及P27蛋白表达呈负相关(r=0.449,P<0.05)。结论:Ki-67及P27的表达可能与DLBCL免疫学分型有关,non-GCB中,Ki-67高表达,P27蛋白低表达。

Objectives To investigate the expression and significance of Ki-67 and P27 in germinal centre B-cell like （GCB） and non-germinal centre B-cell like （non-GCB） of the diffuse large B-cell lymphomas （DLBCL）. Methods A tissue chip was constructed containing 60 cases of DLBCL and 19 cases of reactive hyperplasia of lymph nodes. The subtype of DLBCL including GCB and non- GCB and the expression of Ki-67 and P27 protein were studied. Results （1） In 60 cases of DLBCL, the type of GCB was 29 cases （48.3%）, the type of non-GCB was 31 cases （51.7%）. （2） Ki-67 and P27 protein expression were significant difference in GCB and non-GCB subtypes of DLBCL （P 〈 0.05）. （3） There were negative correlation between Ki-67 and P27 protein expression in DLBCL.