摘要
目的:探讨羊膜对激光角膜光学切除术(Photorefractive keratectomy PRK)后角膜组织TGF-β_1表达的影响。方法:对6只新西兰白兔双眼行PRK,术后1眼立即行角膜表面羊膜移植术,另眼作为对照。术后4周用原位杂交方法检测角膜上皮和基质TGF-β_1mRNA的表达。结果:PRK后4周对照组角膜上皮和基质有较浓密的TGF-β_1mRNA表达,羊膜移植组角膜上皮和基质亦有TGF-β_1mRNA表达,其表达程度较对照组轻(P<0.05)。结论:羊膜移植能降低PRK后角膜组织TGF-β_1mRNA的表达。眼科学报1999;15:215-217。
Objective :To investigate the effect of amniotic membrane on TGF-β1 expression after photorefractive keratectomy (PRK).
Methods: PRK was performed bilaterally on 6 New Zealand white rabbits. One eye was randomly tranplanted with preserved human amniotic membrane, and the other eye served as the control. The expression of TGF-β1 mRNA was detected with in situ hybridization at the 4th weeks after PRK.
Results: TGF-β1mRNA expression was present in the corneal epithelium and stroma at the 4th weeks after PRK. The cornea in amniotic membrane transplanted group had also TGF-@1 mRNA expression, and the expression extent was less than that in control group (P <0. 05).
Conclusion: Amniotic membrane can reduce the expression of TGF-β1 mRNA in cornea after PRK. Eye Science 1999; 15: 215 - 217.
出处
《眼科学报》
1999年第4期215-217,235,共4页
Eye Science
基金
国家自然科学基金资助(批准号:39940007)
关键词
羊膜移植
激光角膜切除术
TGF-Β1
角膜伤口愈合
amniotic membrane, photorefractive keratectomy, cornea, transforming growth factor-β1
