锥兰联合茜素红活细胞染色法观察角膜中央碱烧伤内皮细胞愈合过程

Observation of the Endothelial Healing in Rabbit Corneal Alkali Wounds by Alizarin Red S-trypan Blue Staining Method

目的:研究角膜碱烧伤内皮细胞的愈合过程。方法:锥兰联合茜素红内皮细胞染色法对角膜中央碱烧伤内皮细胞形态学变化进行观察。结果:伤后20分钟内皮细胞已破坏。72小时,损伤区的周边内皮细胞变形向创面内迁移。第7天后缺损区大部分范围均可见梭形细胞覆盖。结论:兔碱烧伤损伤区内皮细胞的修复由邻近内皮细胞变形,增殖和移行长入创面内完成,修复的内皮细胞具有纤维细胞特征。眼科学报1999;15:218-220。

Purpose: To study the healing of the corneal endothelial cells in rabbit corneal alkali wounds. Methods: 26 New Zealand white rabbits were used. A alkali wound was created with a round filter paper, 8 mm in diameter, which was soaked in 0. 5 N NaOH for 5 seconds and was placed centrally on the cornea for 60 seconds. The rabbits were killed in 20 minutes; 1, 2, 6, 12h; 1, 2, 3,5,7, 10, 14 , 30 days after injury. The corneal endotheli-um was examined in the light microscope using a lizarin red S-trypan blue staining method. Results: Twenty minutes after the wound, the corneal endothelial cells were destroyed. 72 hours following the wound, the endothelial cells surrounding the burns became larger, irregular and started to migrate onto the denuded descemet s membrane. The endothelial cells covered the burn with the spindle-shaped cells 7 days after wound. Conclusion : The endothelial damage is healed by the division and migration of remaining endothelial cells at the margin of the wound, which has some characters of fibrob-last. Eye Sciene 1999; 15: 218 - 220.