新生大鼠海马神经元的改良培养

MPROVED CULTURE OF HIPPOCAMAL NEURONS OF NEONATAL RAT

目的:探讨体外培养高纯度神经元的方法。方法:体外培养新生大鼠海马神经干细胞,再使神经干细胞在无血清N2添加剂培养基条件下向神经元方向分化。结果:从新生大鼠海马组织培养出的神经干细胞,表达巢蛋白,神经干细胞的分化细胞绝大部分表达MAP2,极少数几个细胞表达GFAP。结论:通过本实验方法可获得高纯度的神经元,为后续实验奠定基础。

To investigate the method of obtaining high content of neuron in vitro.Methods： Hip-pocamal neural in serum-free pressed nestin, pressed GFAP. periment,which stem cells（NSCs） of neonatal rat were cultured in vitro, and then differentiated to neuron direction medium containing N2 supplement. Results： The NSCs from hippocampus of neonatal rat ex- and most differentiated ceils of NSCs expressed MAP2,sporadically several differentiated cells ex- Conclusion： Highly purified neuron can be obtained by the method adopted by authors in the ex- underlies luther investigation.