威灵仙皂苷对HL-60细胞生长抑制作用的研究

STUDY ON CLEMATIS SAPONIN IN HIBITING HL-60 CELL GROWTH

目的:观察中药威灵仙皂苷对人髓系白血病细胞株HL-60生长抑制的作用,并探讨其可能的作用机制,为开发新的抗肿瘤药物提供理论依据,为白血病患者能进一步提高临床疗效,改善生活质量,延长生存时间做探讨。方法:威灵仙皂苷为本实验室根据文献自行提取,运用细胞培养;Wright's-Giemsa染色各组HL-60细胞,在光学显微镜下观察细胞形态变化并照相;CCK-8的方法观察不同浓度不同时间威灵仙皂苷对急性白血病HL-60细胞的增殖抑制,对比HL-60细胞在加入不同浓度威灵仙皂苷和作用不同时间后的生长情况和细胞活性并与三氧化二砷作用进行比较,探讨威灵仙皂苷对急性白血病细胞生长抑制的作用。结果:加入不同浓度的威灵仙皂苷作用后,急性白血病细胞受到不同程度的抑制,且呈浓度和时间的依赖性,经形态学检查发现细胞体积变小,细胞核高度浓缩聚集,甚至断裂形成凋亡小体。结论:实验证明威灵仙皂苷有抑制急性白血病细胞生长的作用,且呈浓度和时间的依赖性;高浓度的威灵仙皂苷与三氧化二砷的作用无统计学意义。

To observe the inhibition induced by Chinese medicine clematis saponin against human myeloid leukemia cell line HL-60 and investigate the potential mechanism, M^thods： Acute leukemic cells were treated by different concentration of clematis saponin for elifferent period of time： The morphology and growth activity were observed. Results：HL-60 cells were inhibited by clematis saponin in a time and concentration de- pendent manner . By means of morphology examination , cell volume shrinked and nucleus was highly crimpled and aggregated, and even broke to form apoptotic body. Conclusion： This experiment proves that clematis saponin can inhibit the growth of acute leukemic cell ,inhibition effect of high concentration clematis sapanin has no significant difference with that of arsenic trioxide.