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HDACS抑制剂对人肝癌细胞株HepG2作用的研究 被引量:1

STUDY OF THE EFFECT OF HDACS INHIBITOR——TRICHOSTATIN A ON HUMAN HEPATOCELLULAR CARCINOMA HEPG2 CELL LINE
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摘要 目的:通过组蛋白去乙酰化酶(histone deacetylase,HDACS)抑制剂古抑菌素A(Trichostatin A,TSA)对人肝癌细胞株HepG2(以下简称HepG2)和正常肝细胞株LO2(以下简称LO2)增殖与凋亡作用的比较,探讨TSA对肝癌的作用机制。方法:应用光学显微镜、透射电镜、四氮唑蓝(MTT)法、脱氧核苷酸末端转移酶介导的dTP缺口末端标记技术(TUNEL法)、免疫细胞化学法观察经不同浓度TSA处理后的HepG2和LO2的增殖、凋亡与凋亡相关蛋白的改变。结果:(1)HepG2细胞经TSA处理后,电镜下超微结构发生了凋亡早期改变。(2)小剂量TSA(250nmol/L)对HepG2细胞具有较强的生长抑制作用,对LO2细胞影响不明显,当TSA浓度达到1000nmol/L以上时,对LO2表现出明显的细胞毒性作用。(3)TSA可诱导HepG2细胞凋亡,并增加凋亡相关蛋白Bax的表达。结论:TSA可明显抑制肝癌细胞HepG2的增殖,其机制可能与上调Bax的表达,诱导细胞凋亡有关。 To investigate the mechanism of trichostatin A (TSA) by comparing its effects on prolif- eration and apotosis of human hepatocellular carcinoma HepG2 cellline and normal hepatocellular LO2 cellline. Methods:MTY, Phase-contrast-microscopy, electron-microscopy, terminal deoxynucleotidyl transferase TdT-medi- ated dUTP-biotin nick end labeling (TUNEL), and immunocytochemistry were conducted to observe the alteration of cell line proliferation, apotosis and apoptosis-related protein of HepG2 and LO2 after treated with TSA.Results:(1) The HepG2 cellline altered their cell line forms and the early apoptosis was observed under electron microscope. (2) Low dosage TSA (250nmol/L) had a very strong inhibition to HepG2 cellline, nearly with no effect on LO2 cellline. When the TSA concentration reached 1000nmol/L or above, apparent cytotoxicity appeared to LO2 cell line. (3)TSA can induce the apoptosis of HepG2 cell line and increase the expressions of Bax. Conclusion: TSA can inhibit the proliferation of HepG2 cell line. This may be related to cell apoptosis by upmodulating the expression of Bax.
出处 《泸州医学院学报》 2011年第3期244-247,共4页 Journal of Luzhou Medical College
基金 四川省卫生厅科研项目(No.060065)
关键词 肝癌 组蛋白去乙酰化酶 古抑菌素A 细胞凋亡 Hepatoma Histone deacetylase TSA Apoptosis
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