西安地区丙型肝炎病毒基因分型研究

Study on hepatitis C virus genotyping in Xi'an area

目的 了解西安地区不同人群ＨＣＶ 基因型分布．方法 先用逆转录－ 套式多聚酶链反应检测抗－ ＨＣＶ 阳性血清的ＲＮＡ， 阳性者再用限制性片段长度多态性分析法进行ＨＣＶ 基因分型．结果 １１３ 份ＨＣＶＲＮＡ 阳性血清的基因型如下：输血后丙肝组，Ⅱ型、Ⅲ型及Ⅱ／ Ⅲ混合型分别为８０ ％ ，１６－７ ％ 和３－３ ％ ．散发性丙肝组，Ⅱ型、Ⅲ型分别为７８－９ ％ 和２１－１ ％ ，未检出Ⅱ／Ⅲ混合型． 肝硬变组， Ⅱ型、Ⅲ型及Ⅱ／ Ⅲ混合型分别为８７－９ ％ ，９－１ ％ 和３ ％ ． 原发性肝癌组，Ⅱ型、Ⅲ型分别为８７－１ ％和１２－９ ％ ，未检出Ⅱ／ Ⅲ混合型． 分型总结果为：ＨＣＶⅡ型、Ⅲ型及Ⅱ／ Ⅲ混合型分别为８４－１ ％ ，１４－２ ％ 和１－７ ％ ．结论 ＨＣＶⅡ型是西安地区优势株． 不同人群中ＨＣＶ 基因型总体分布差异无显著性．

AIM To investigate the distribution of HCV genotypes in different populations in Xi'an area. METHODS Anti HCV positive serum from 131 patients were detected for HCV RNA by reverse transcription polymerase chain reaction (RT PCR). HCV RNA positive sera (113 samples) were determined for their genotypes by the restriction fragment length polymorphism (RFLP) analysis of PCR products of 5' NC region. RESULTS Genotypes of the 113 HCV RNA positive serum were analyzed. In post transfusion hepatitis C group, HCV Ⅱ, HCV Ⅲ and HCV Ⅱ/*!Ⅲ were 24(80%), 5(16 7%) and 1(3 3%) respectively. In sporadic hepatitis C group, 15(78 9%) were HCV Ⅱ and 4(21 1%) were HCV Ⅲ. In cirrhosis group, HCV Ⅱ, Ⅲ and Ⅱ/*!Ⅲ were 29(87 9%), 3(9 1%) and 1(3%) respectively. In primary hepatocarcinoma group, there were 27(87 1%) HCV Ⅱ and 4(12 9%) HCV Ⅲ. Overall, HCV Ⅱ, HCV Ⅲ and HCV Ⅱ/*!Ⅲ were 95(84 1%), 16(14 2%) and 2(1 7%) respectively. CONCLUSION Type Ⅱ is predominant HCV genotype in Xi'an area. The overall distribution of HCV genotypes in different populations had no significant difference ( P >0 05) .