反相高效液相色谱法测定化妆品中的酮麝香

Determination of Musk Ketone Content in Cosmetics by Reversed-phase High Performance Liquid Chromatography

酮麝香是化妆品中的限用物质,对人体有致癌作用,因此,有必要对其含量进行分析。采用超声辅助提取法对不同基质类型的化妆品样品进行预处理。利用反相高效液相色谱法测定化妆品中的酮麝香。色谱条件为Kromasil C18(250mm×4.6mm i.d.,5μm)色谱柱,流动相V(乙腈)﹕V(水)=80﹕20,流速1.0mL/min,检测波长235nm。结果表明,在此条件下,酮麝香在0.5-150μg/mL范围内与相应的峰面积具有良好的线性关系(相关系数r为0.9998),线性回归方程为A=13500ρ-4829,回收率为90.5%-102.5%,RSD为0.45%-0.98%。因此,研究建立的利用反相高效液相色谱法测定化妆品中的酮麝香的方法准确、可靠、适用性广,可为酮麝香的分析及国家相关法规的制定提供一定的参考。

Due to the human carcinogenic effect of musk ketone it is the restriction ingredient in cosmetics,and the content analysis of musk ketone is necessary.The various matrixes of cosmetics are prepared by using ultrasonic-assisted extraction.A reversed-phase high performance liquid chromatography method for the determination of musk ketone in cosmetics is established.The samples are analyzed under the condition of Kromasil-C 18 column（250mm×4.6mm i.d.,5μm） with UV 235nm.The mobile phase is a gradient elution with V（CH 3 CN）：V（H 2 O） = 80：20 at a flow rate of 1.0mL/min.Results show that as the concentration of musk ketone is in the range of 0.5 — 150μg/mL,the relationship between concentration and the corresponding peak area presents the good linearity（r=0.9998）.The regression equations could be expressed as A=13500ρ-4829;and the recovery ratio of musk ketone achieves at 90.5% —102.5%.The RSD of the method is 0.45% —0.98%.Therefore,an accurate and stabilizing method with extensive applications is proposed and built.The method provides the reference for the analysis on musk ketone and for the establishment of the state regulations.