建立人同种异体心脏瓣膜库及心瓣制备的临床应用

EXPERIENCE OF BANKING AORTIC VALVE HOMOGRAFTS AND CLINICAL APPLCATION

总结分析了我院心脏瓣膜库的建立、制备、管理及临床应用。于 1987年建立心脏瓣膜库 ,并制定了供体标准、采集处理贮存全过程中的质量监测标准 ,以保证临床应用的安全性。在无菌条件下采集 ,Hank′s液中修剪 ,RPMI 16 4 0加抗生素过渡 ,液氮贮存。 1987年 9月至 1997年 12月 ,共采集处理贮存同种心脏瓣膜 110份 ,临床应用 2 0份 ,其中我院 11份 ,外院 9份 ,取得较满意的效果。

Banking aortic valve homografts were performed in our hospital for safeguarding vecipients.The guidelines of donor selection were formulated and quality control was applied during precurement,preparation and storage of the grafts.Aortic valve homografts were harvested under aseptic, treated in Hank's and RPMI 1640 with antibiotic, cryopreserved in Liquid Nitrogen.From September 1987 to December 1997,altogether 200 aortic valves were harvested,110 of them were cryopreserved.20 vlaves were implanted in our hospital and other hospital with satisfactory results.