疏肝健脾饮对糖尿病性胃轻瘫大鼠的干预作用

Intervention of ShuGanJianPi Decoction on diabetic gastroparesis rats:Experimental study

目的观察疏肝健脾饮对链脲佐菌素致糖尿病性胃轻瘫大鼠的胃肠推进指标及nNOS、5-HT4R、β2-AR等指标的影响并探讨其作用机制。方法以2%链脲佐菌素(STZ)加不规则饮食4周造成糖尿病性胃轻瘫大鼠模型,将30只模型大鼠随机分为模型组和中药组,4周后中药组以相当于成人临床常用剂量的2倍灌胃给药,模型组灌胃等剂量生理盐水,1次/d,给药4周。另取10只正常大鼠作为正常组。实验通过胃肠推进指标和nNOS、5-HT4R、β2-AR免疫组化的结果观察疏肝健脾饮对糖尿病性胃轻瘫大鼠的干预作用。结果疏肝健脾饮不仅降低糖尿病性胃轻瘫大鼠的血糖,还可以明显改善胃肠推进,并改善nNOS、5-HT4R、β2-AR等指标,与模型组比较有显著性差异(P<0.05)。结论疏肝健脾饮能降低糖尿病性胃轻瘫大鼠的血糖,加速胃排空,并改善nNOS、5-HT4R、β2-AR等免疫组化指标。

Objective To observe the effects of ShuGanJianPi Decoction in rat model with diabetic gastroparesis（DGP） on the gastrointestinal propulsion,nNOS,5-HT4R and β2-AR.Method The rats with diabetic gastroparesis induced by injecting 2% STZ and giving irregular diet continuing for 4 weeks,30 diabetic gastroparesis rats were divided into two groups randomly：the treatment group and the control group.Then,the treatment group was given ShuGanJianPi Decoction continuing for 4 weeks at the mount of 2 times of adult common dose.Other groups was given NS of the same mount,once daily,continuing for 4 weeks.Authors chosed 10 rats as the normal group.Then the results of gastrointestinal propulsion,nNOS,5-HT4R and β2-AR were observed.Result The treatment group had better effects than the control group in lowering the blood sugar,improving the level of gastrointestinal propulsion and 5-HT4R,lowering the content of nNOS（P0.01）.And there was significant difference between the treatment group and control group（P0.05）.Conclusion ShuGanJianPi Decoction has obvious effects in lowering the blood glucose,improving the level of gastrointestinal propulsion and 5-HT4R,and lowering the lever of nNOS.