摘要
目的：了解黄芪多糖对体外培养成骨细胞增殖、分化能力的影响。方法：采用水煮醇沉法提取黄芪多糖，用ＴＬＣ进行定性，用比色法定量，用含高低两种剂量（０．５ｍ ｇ／ｍ ｌ，５．０ｍ ｇ／ｍ ｌ）的黄芪多糖的培养液体外培养成骨细胞，ＭＴＴ法及ＡＬＰ比活性测定观察成骨细胞增殖率及细胞活性变化。结果：低浓度黄芪多糖（０．５ｍ ｇ／ｍ ｌ）及高浓度黄芪多糖（５．０ｍ ｇ／ｍ ｌ）短期时（２ 天）促进成骨细胞增殖；高浓度（５．０ｍ ｇ／ｍ ｌ）长期（４ 天）抑制成骨细胞增殖，降低其活性。结论：黄芪多糖对成骨细胞增殖。
Objective:To understand the effect of APS on the osteogenic capacity of osteoblasts in vitro.Methods:APS was refine with water and alcoholysis,qualitatively analysis with TLC and quantitatively analysis with colorimetric analysis. Osteoblast were cultured in vitro with APS of 0.5mg/ml and 5.0mg/ml. MTT and ALP actives were used to observed the effect of APS on osteoblasts proliferation rate and osteogenic capacity.Results:APS concentration of 0.5mg/ml and 5.0mg/ml can improve the proliferation rate and osteogenic capacity through short cultured (2day) and 5.0mg/ml can decrease these effect through long cultured (4day) of normal osteoblasts.Conclusion:APS has two-way regular capacity on the osteogenic capacity of osteoblasts in vitro.
出处
《中国中医骨伤科》
1999年第6期1-4,共4页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
上海市卫生局科研基金项目!编号:96198
