柑橘衰退病和碎叶病复合感染病原的脱毒研究

Eradicating double infected pathogen of CTV and CTLV in Citrus

以带毒柑橘品种为试材,采用植物中药制剂处理与热处理结合、茎尖微芽嫁接与植物中药制剂处理结合,用RT-PCR法检测病原带毒情况,并在药剂处理后1、3、5、7、9 d取样,检测抗性相关酶活性的含量变化,结果表明:采用板蓝根和黄芩混合中药制剂处理8次,结合热处理,在PC板制成的温室(白天40℃(6 h/d),晚上25℃,处理50 d)中,可有效地脱除试验苗木中柑橘衰退病和柑橘碎叶病复合感染病原;对柑橘茎尖微芽嫁接苗进行板蓝根和黄芩混合中药制剂脱毒处理后苗木经RT-PCR法检测表明:85.7%植株可以脱除柑橘衰退病和柑橘碎叶病病毒;药剂处理后PPO、POD、SOD酶活性与对照相比均有所提高。

A new method for eradicating the double infected pathogen of CTV and CTLV in citrus was explored in this experiment.The citrus plants were selected from those conserved in net greenhouse of National Indoor Conservation Center of Virus-free Germplasms of Fruit Crops,Huazhong Agricultural University.The infection of pathogen was detected with reverse transcripts PCR（RT-PCR）.Results showed that the suitable protocol was using the treatment of the antiviral medicament with Chinese medicines（eight times） and combinated with heat treatment of the highest temperature at 40℃ during the day（6 h/d）,the temperature at 25℃ in the evening kept for fifty days in the PC made greenhouse.The protocol mentioned above was proved to completely eradicate double infected pathogen of CTV and CTLV;Furthermore,the combined treatment of shoot-tip grafting and the antiviral Chinese medicines was identified 85.7% eradication of the double pathogen.In above experiments,the leaves were sampled at 1,3,5,7,9 d after the Chinese medicines treatment.The results showed that the activity of the resistance related enzymes PPO,POD,SOD were increased compared with the control.